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Culture media and sources of nitrogen promoting the formation of stromata and ascocarps in Petromyces alliaceus (Aspergillus section Flavi)

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Abstract:

Petromyces alliaceus Malloch and Cain is the only known sexually reproducing fungus classified in Asper gillus section Flavi. The goal of this research was to identify culture media and sources of nitrogen that best support the formation of stromata with ascocarps. Three cultures of P. alliaceus isolated from crop field soils were grown on selected agar media in Petri dishes for 7 months at 30 °C in darkness. The largest numbers of stromata were recorded for cultures grown on Czapek's agar (CZA) and a mixed cereal agar (MCA), while the percentage of stromata containing ascocarps was greatest (P ≤ 0.05) for cultures grown on MCA (25%–28%). When P. alliaceus was grown on standard CZA containing 0.3% NaNO3, only 5% of the stromata contained ascocarps. A greater percentage of the stromata (15%–22%) formed ascocarps when the NaNO3 in CZA was replaced with an equivalent amount of available nitrogen supplied by ammonium tartrate, glutamic acid, or serine.Key words: ascospores, homothallic, sclerotia, essential amino acids.

Petromyces alliaceus Malloch et Cain est le seule champignon connu à reproduction sexuée classifié dans les Aspergillus section Flavi. Le but de cette recherche été d'identifier les milieux de culture et la source d'azote soutenant le mieux la formation de stroma avec ascocarpes. Trois cultures de P. alliaceus isolées de sols agricoles furent cultivées sur les milieux gélosés sélectionnés dans des boîtes de Petri pendant 7 mois à 30 °C dans l'obscurité. Le plus grand nombre de stroma fut obtenu chez des cultures cultivées sur de l'agar de Czapek (CZA) et un agar de céréales mixtes (MCA) alors que le pourcentage de stroma contenant des ascocarpes était le plus élevé (P ≤ 0,05) pour des cultures cultivées sur du MCA (25 % – 28 %). Lorsque P. alliaceus fut cultivé sur du CZA normal contenant 0,03 % de NaNO3, seulement 5 % des stroma contenaient des ascocarpes. Un pourcentage supérieur de stroma (15 % – 22 %) ont formé des ascocarpes lorsque le NaNO3 dans le CZA a été remplacé par une quantité équivalente d'azote disponible fourni par du tartrate d'ammonium, de l'acide glutamique ou de la sérine.Mots clés : ascospores, homothallique, sclérote, acide aminé essential.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2005-09-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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