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Listeria monocytogenes listeriolysin O and phosphatidylinositol-specific phospholipase C affect adherence to epithelial cells

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Listeria monocytogenes, a foodborn intracellular animal and human pathogen, produces several exotoxins contributing to virulence. Among these are listeriolysin O (LLO), a pore-forming cholesterol-dependent hemolysin, and a phosphatidylinositol-specific phospholipase C (PI-PLC). LLO is known to play an important role in the escape of bacteria from the primary phagocytic vacuole of macrophages, and PI-PLC supports this process. Evidence is accumulating that LLO and PI-PLC are multifunctional virulence factors with many important roles in the host-parasite interaction other than phagosomal membrane disruption. LLO and PI-PLC may induce a number of host cell responses by modulating signal transduction of infected cells via intracellular Ca2+ levels and the metabolism of phospholipids. This would result in the activation of host phospholipase C and protein kinase C. In the present study, using Bacillus sub tilis strains expressing LLO, PI-PLC, and simultaneously LLO and PI-PLC, we show that LLO and PI-PLC enhance bacterial binding to epithelial cells Int407, with LLO being necessary and PI-PLC playing an accessory role. The results of this work suggest that these two listerial proteins act on epithelial cells prior to internalization.Key words: Listeria monocytogenes, listeriolysin O, phosphatidylinositol-specific phospholipase C, Bacillus subtilis, adherence.

Listeria monocytogenes, un pathogène intracellulaire animal et humain de source alimentaire, produit plusieurs exotoxines contribuant à la virulence. On retrouve parmi celles-ci la listériolysine O (LLO), une hémolysine formant des pores dépendants du cholestérol, et une phospholipase C spécifique au phosphatidylinositol (PI-PLC). LLO est reconnu comme un important acteur dans la fuite de la bactérie de la vacuole phagocytaire primaire des macrophages et la PI-PLC soutient ce processus. De plus en plus de preuves indiquent que la LLO et la PI-PLC sont des facteurs de virulence multifonctionnels jouant plusieurs rôles importants dans l'interaction hôte-parasite autres que la déchirure de la membrane phagosomale. La LLO et la PI-PLC pourraient induire une variété de réponses de la cellule-hôte en modulant la transduction des signaux des cellules infectées à travers les niveaux de Ca2+ intracellulaire et le métabolisme des phospholipides. Ceci entraînerait l'activation de la phospholipase C de l'hôte et la protéine kinase C. Dans l'étude présente, en utilisant des souches de Bacillus subtilis exprimant LLO, PI-PLC, et LLO et PI-PLC simultanément, nous avons démontré que la LLO et la PI-PLC favorisent l'attachement des bactéries aux cellules épithéliales Int407, LLO étant nécessaire et PI-PLC jouant un rôle accessoire. Les résultats de ces travaux indiquent que ces deux protéines listériales agissent sur les cellules épithéliales avant l'internalisation.Mots clés : Listeria monocytogenes, listériolysine O, phospholipase C spécifique au phosphatidylinositol, Bacillus subtilis, adhérence.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: September 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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