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The role of clp-regulated factors in antagonism against Magnaporthe poae and biological control of summer patch disease of Kentucky bluegrass by Lysobacter enzymogenes C3

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Abstract:

A global regulator was previously identified in Lysobacter enzymogenes C3, which when mutated, resulted in strains that were greatly reduced in the expression of traits associated with fungal antagonism and devoid of biocontrol activity towards bipolaris leaf-spot of tall fescue and pythium damping-off of sugarbeet. A clp gene homologue belonging to the crp gene family was found to globally regulate enzyme production, antimicrobial activity, and biological control activity expressed by Lysobacter enzymogenes C3 (Kobayashi et al. 2005). Here, we report on the expansion of the biocontrol range of L. enzymogenes C3 to summer patch disease caused by Magnaporthe poae. The clp mutant strain 5E4 was reduced in its ability to suppress summer patch disease compared with the wild-type strain C3 and was completely devoid of antifungal activity towards M. poae. Furthermore, cell suspensions of 5E4 were incapable of colonizing M. poae mycelium in a manner that was distinct for C3. Strain C3 demonstrated biosurfactant activity in cell suspensions and culture filtrates that was associated with absorption into the mycelium during the colonization process, whereas 5E4 did not. These results describe a novel interaction between bacteria and fungi that intimates a pathogenic relationship.Key words: lytic enzymes, biosurfactant, turfgrass biocontrol agent, mycopathogenic bacteria.

Un régulateur global codé par le gène clp fut identifié précédemment chez la souche C3 de Lysobacter enzymogenes, qui lorsque muté a entraîné la génération de souches manifestant une réduction profonde de l'expression de traits associés à l'antagonisme envers les champignons et dépourvues d'activité de biocontrôle envers la tacheture foliaire de bipolaris chez la fétuque élevée et envers la fonte des semis de pythium chez la betterave à sucre. Un homologue du géne clp appartenant B la famille du géne crp régule globalement la production d'enzymes lytiques, l' activité antimicrobienne et l'activité de biocontrôle exprimées par la souche C3 de Lysobacter enzymogenes (Kobayashi et al. 2005). Nous décrivons dans ce rapport l'expansion du spectre de biocontrôle de la souche C3 de L. enzymogenes à la maladie de la tache d'été causée par Magnaporthe poae. La souche 5E4 mutante pour clp a démontré une réduction de la suppression de la tache d'été comparativement à la souche sauvage C3 et était complètement dépourvue d'activité antifongique envers M. poae. De plus, à la différence de la souche C3, des suspensions cellulaires de 5E4 furent incapables de coloniser les mycélium de M. poae. Les suspensions cellulaires et les filtrats de culture de la souche C3 ont démontré une activité de biosurfactant qui fut associée avec l'adsorption dans le mycélium durant le processus de colonisation, une activité qui ne fut pas retrouvée chez 5E4. Ces résultats décrivent une nouvelle interaction entre les bactéries et les champignons qui suggèrent une relation pathogène.Mots clés : enzymes lytiques, biosurfactants, agent de biocontrôle du gazon, bactéries mycopathogènes.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2005

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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