Skip to main content

In vitro inhibition of Helicobacter pylori by Enterococcus faecium GM-1

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

A strain of Enterococcus faecium that exhibits antibacterial activity against Helicobacter pylori was isolated from the feces of newborn babies. This strain was selected for its ability to inhibit the growth of H. pylori and to withstand harsh environmental conditions, such as acidic pH and high bile concentration. Biochemical tests and 16S rRNA sequencing specific for Enterococcus faecium GM-1 were used to identify the isolated bacterial strain. In vitro studies were used to investigate the inhibitory effects of E. faecium GM-1 on H. pylori. These results showed that the culture supernatant of E. faecium GM-1 significantly decreased the viability and urease activity of H. pylori. This inhibitory activity remained after adjustment of pH of culture supernatant to neutral. However, treatment with proteolytic enzymes reduced the anti-H. pylori activity of GM-1. Therefore, some substance(s) of E. faecium GM-1 other than pH and lactic acid might be associated with this inhibitory activity. Analysis by electron microscopy also demonstrated that the addition of GM-1 destroyed the cell structure of H. pylori. Additional studies suggested that the binding of H. pylori to human colonial cells decreased in the presence of GM-1.Key words: Enterococcus faecium, Helicobacter pylori, inhibition, human fecal strain, proteinaceous substance(s).

Une souche de Enterococcus faecium démontrant une activité antibactérienne contre Helicobacter pylori fut isolée des selles de nouveau-nés. Cette souche fut sélectionnée pour sa capacité à inhiber la croissance de H. pylori et à résister à des conditions environnementales difficiles, tel qu'un pH acide et une concentration élevée de bile. Des tests biochimiques et le séquençage de l'ARNr 16S spécifique à Enterococcus faecium GM-1 furent employés pour identifier la souche bactérienne isolée. Des études in vitro furent conduites afin d'évaluer les effets inhibiteurs de E. faecium GM-1 sur H. pylori. Ces résultats montrent que le surnageant de culture de E. faecium diminue significativement la viabilité et l'uréase de H. pylori. Cette activité inhibitrice fut conservée après l'ajustement du pH du surnageant de culture à neutralité. Toutefois, un traitement avec des enzymes protéolytiques a diminué l'activité anti-H. pylori de GM-1. Par conséquent, une ou des substances de E. faecium GM-1 autre que le pH et l'acide lactique pourraient être associées avec cette activité inhibitrice. L'analyse par microscopie électronique a également démontré que l'ajout de GM-1 détruisait la structure cellulaire de H. pylori. Des études supplémentaires ont suggéré que la liaison de H. pylori à des cellules humaines du côlon a diminué en présence de GM-1.Mots clés : Enterococcus faecium, Helicobacter pylori, inhibition, souche humaine fécale, substances protéiques.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2005

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • Ingenta Connect is not responsible for the content or availability of external websites
nrc/cjm/2005/00000051/00000008/art00002
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more