Cytochrome aa3 in facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense

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Abstract:

We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N′, N′-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c oxidase activity. These enzymatic activities of cytochrome aa3 were inhibited by cyanide and azide. This cytochrome contained heme As, but not typical heme A. An analysis of trypsin-digested fragments indicated that 1 subunit of this cytochrome was identical to the gene product of subunit I of the SoxM-type heme – copper oxidase (poxC). This is the first report of a terminal oxidase in hyperthermophilic crenarchaeon belonging to the order Thermoproteales.Key words: aerobic respiratory chain, terminal oxidase, Archaea, hyperthermophile, Pyrobaculum.

Nous avons partiellement purifié et caractérisé le cytochrome aa3 de l'archéen anaérobie facultatif et hyperthermophile Pyrobaculum oguniense. Ce cytochrome aa3 a démontré une activité de consommation d'oxygène avec le N, N, N′, N′-tetramethyl-1,4-phenylenediamine et l'ascorbate comme substrats et a également montré une activité cytochrome c oxydase bovine. Ces activités enzymatiques du cytochrome aa3 furent inhibées par le cyanure et l'azide. Ce cytochrome contenait l'hème As mais non l'hème A typique. Une analyse des fragments digérés par la trypsine a indiqué qu'une sous-unité de ce cytochrome était identique au produit du gène de la sous-unité I de la hème – cuivre oxydase de type SoxM (poxC). Il s'agit de la première mention d'une oxydase terminale chez les crenarchéens hyperthermophiles appartenant à l'ordre Thermoproteales.Mots clés : chaîne respiratoire aérobie, oxydase terminale, archéens, hyperthermophiles, Pyrobaculum.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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