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Virulence deficiency caused by a transposon insertion in the purH gene of Xanthomonas oryzae pv. oryzae

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Abstract:

Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. We have identified a Tn5-induced virulence-deficient mutant (BXO1704) of X. oryzae pv. oryzae. The BXO1704 mutant exhibited growth deficiency in minimal medium but was proficient in inducing a hypersensitive response in a non-host tomato plant. Sequence analysis of the chromosomal DNA flanking the Tn5 insertion indicated that the Tn5 insertion is in the purH gene, which is highly homologous to purH genes of other closely related plant pathogenic bacteria Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris. Purine supplementation reversed the growth deficiency of BXO1704 in minimal medium. These results suggest that the virulence deficiency of BXO1704 may be due to the inability to use sufficient purine in the host.Key words: auxotroph, plant pathogen, resistance.

Xanthomonas oryzae pv. oryzae entraîne le dépérissement foliaire bactérien, une maladie grave du riz. Nous avons identifié un mutant de X. oryzae pv. oryzae à virulence déficiente (BXO1704) provoquée par le Tn5. Le mutant BXO1704 démontrait un défaut de croissance dans un milieu minimal mais était compétent à induire une réponse d'hypersensibilité chez une plante non hôte, la tomate. L'analyse de la séquence de l'ADN chromosomique flanquant l'insertion du Tn5 a indiqué que l'insertion du Tn5 se retrouvait dans un gène nommé purH qui est hautement homo logue aux gènes purH d'autres bactéries pathogènes de plantes apparentées: Xanthomonas axonopodis pv. citri et Xanthomonas campestris pv. campestris. L'ajout d'un supplément de purine a rectifié le défaut de croissance de BXO1704 dans un milieu minimal. Ces résultats indiquent que la virulence déficiente de BXO1704 serait due à l'incapacité d'extraire suffisamment de purine de l'hôte.Mots clés : auxotrophe, pathogènes des plantes, résistance.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: July 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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