Antifungal susceptibility and genotypical pattern of Microsporum canis strains

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Abstract:

Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophytes to antifungals. The aim of this study was to evaluate the possible correlation between the antifungal susceptibility and genotypical pattern of Microsporum canis strains isolated in dogs and cats with dermatophytosis in Northeast Brazil. The antifungal susceptibility study was conducted using the broth microdilution test with griseofulvine, ketoconazole, itraconazole, and fluconazole. The genotypical analysis was performed using the RAPD method. The antifungal susceptibility analysis showed that all the strains of M. canis analyzed (n = 22) were sensitive to griseofulvine (0.25 µg/mL ≤ minimum inhibitory concentration (MIC) ≤ 1 µg/mL), ketoconazole (0.25 µg/mL ≤ MIC ≤ 2 µg/mL), itraconazole (0.25 µg/mL ≤ MIC ≤ 1 µg/mL), and fluconazole (1 µg/mL ≤ MIC ≤ 16 µg/mL). The RAPD results showed that all analyzed strains are genetically similar. Thus, based on antifungal susceptibility analysis and RAPD data, a possible correlation can be shown between the antifungal susceptibility and the genotypical pattern of the strains of M. canis from Northeast Brazil.Key words: Microsporum canis, antifungal susceptibility testing, RAPD.

Les dermathophytes sont un groupe de champignons qui ont la capacité d'envahir des tissus kératinisés d'humain et d'animaux. L'analyse de la sensibilité in vitro à des antifongiques et des étude génétiques par amplification aléatoire de DNA polymorphique (RAPD) ont été utilisés pour détecter un polymorphisme ainsi que pour mettre en évidence une possible résistance des dermatophytes aux antifongiques. L'objectif cette étude a été d'évaluer la possible corrélation entre la sensibilité aux antifongiques et le pattern génotypique de souches de Microsporum canis isolées de chiens et de chats atteints de dermatophytose, au nord-est du Brésil. L'étude de la sensibilité aux antifongiques a été réalisée en utilisant le test de la microdilution avec 4 drogues antifongiques: fluconazole, itraconazole, cetoconazole et griseofulvine. L'analyse génétique de M. canis a été réalisée par la technique de RAPD. L'analyse de la sensibilité aux antifongiques a démontré que toutes les souches de M. canis analysées (n = 22) étaient sensibles à la griseofulvine (0.25 µg/mL ≤ MIC ≤ 1 µg/mL), au ketoconazole (0.25 µg/mL ≤ MIC ≤ 2 µg/mL), à l'itraconazole (0.25 µg/mL ≤ MIC ≤ 1 µg/mL) et au fluconazole (1 µg/mL ≤ MIC ≤ 16 µg/mL). D'autre part, la technique de RAPD a mis en évidence que toutes les souches analysées étaient génétiquement similaires. Sur base de ces données, une possible corrélation entre la sensibilité aux antifongiques et le pattern génotypique de souches de M. canis provenant du nord-est du Brésil a pu être mise en évidence.Mots clés : Microsporum canis, tests de sensibilité, RAPD.

Document Type: Research Article

Publication date: June 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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