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Substrate specificity of glucose dehydrogenase (GDH) of Enterobacter asburiae PSI3 and rock phosphate solubilization with GDH substrates as C sources

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Abstract:

Enterobacter asburiae PSI3 is a rhizospheric isolate that solubilizes mineral phosphates by the action of a phosphate starvation-inducible GDH (EC 1.1.5.2). We report here that GDH activity of this isolate shows broad substrate range, being able to act on mono and disaccharides. Enterobacter asburiae PSI3 was proficient at bringing about a drop in pH and solubilization of RP with the use of 75 mmol/L of each of the GDH substrate sugars tested as the sole C source. It liberated amounts of P ranging from 450 µmol/L (on arabinose) to 890 µmol/L (on glucose). When grown on a mixture of 7 GDH substrates at concentrations of 15 mmol/L each, the bacterium solubilized RP equivalent to 46% of the value when 75 mmol glucose/L was the C source. HPLC analysis of the culture supernatant under these conditions showed that the acidification of the media is primarily due to the production of organic acids. The significance of these results on the efficacy of E. asburiae PSI3 at solubilizing phosphates under rhizospheric conditions is discussed.Key words: glucose dehydrogenase, substrate specificity, P solubilization, Enterobacter asburiae PSI3.

Enterobacter asburiae PSI3 est un isolat de la rhizosphère qui solubilise les phosphates minéraux par l'action d'une D-glucose déshydrogénase (EC 1.1.5.2) (GDH) induite par la carence en phosphate. Nous rapportons dans cet article que l'activité GDH de cet isolat démontre un spectre de substrats élargi, capable d'agir sur des mono- et des disaccharides. Enterobacter asburiae PSI3 était compétent à diminuer le pH et à solubiliser le phosphate de roche (PR) en utilisant 75 mmol/L de chacun des sucres substrats de la GDH testés comme seule source de carbone et à libérer du P allant de 450 µmol/L (sur de l'arabinose) à 890 µmol/L (sur du glucose). Lorsque cultivés dans un mélange de 7 substrats de GDH à 15 mmol/L chaque, la bactérie a solubilisé l'équivalent de 46 % du PR solubilisé lorsque 75 mmol/L étaient utilisés comme sources de C. Une analyse par HPLC du surnageant de culture dans les conditions mentionnées ci-dessus a montré que l'acidification du milieu était principalement attribuable à la production d'acides organiques. La portée de ces résultats sur l'efficacité de E. asburiae PSI3 à solubiliser les phosphates dans les conditions retrouvées dans la rhizosphère est discutée.Mots clés : glucose déshydrogénase, spécificité du substrat, stabilisation du P, Enterobacter asburiae PSI3.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: June 1, 2005

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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