Skip to main content

The influence of Bacillus subtilis RB14-C on the development of Rhizoctonia solani and indigenous microorganisms in the soil

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

The effect of soil inoculation with an antagonistic strain Bacillus subtilis RB14-C on the development of Rhizoctonia solani and changes occurring in soil and rhizosphere microbial communities were studied. RB14-C was added to the soil as a water suspension of the cells or as a broth culture. Application of cell suspensions to non-planted soil reduced the number of culturable bacteria. The density of R. solani and the number of filamentous fungi were not significantly affected by RB14-C. A similar effect was observed in the rhizosphere of tomato plants growns in bacterized soil. Broth cultures of RB14-C suppressed R. solani 1 d after inoculation, but after 3 d there was no difference in the pathogen density between soil amended with broth culture and control soil. In microcosm studies, cell suspensions of RB14-C also did not inhibit growth of R. solani on filters buried in soil. However, an inhibitory effect was obtained when a broth culture of the bacterium was used. The effect of RB14-C on fungal biomass was also estimated by measurement of ergosterol concentration in soil. It was found that ergosterol was mostly derived from R. solani and that there were no significant differences in its content between untreated soil and soil treated with RB14-C. The results suggest that suppression of Rhizoctonia damping-off by B. subtilis RB14-C probably is not related to the reduction of the pathogen population in the soil.Key words: B. subtilis, R. solani, ergosterol, native microflora.

Nous avons étudié l'impact de l'inoculation du sol avec la souche antagoniste RB14-C de Bacillus subtilis sur le développement de Rhizoctonia solani de même que les changements engendrés dans le sol et les communautés microbiennes de la rhizosphère. RB14-C été ajouté au sol sous forme de suspension cellulaire dans de l'eau ou du bouillon de culture de la bactérie. Le traitement d'un sol non planté avec la suspension cellulaire a diminué le nombre de bactéries cultivables. La densité de R. solani et le nombre de champignons filamenteux n'ont pas été significativement affectés par RB14-C. Un effet semblable fut observé dans la rhizosphère de plants de tomate cultivés dans un sol colonisé. Bouillon de culture de RB14-C a inhibé R. solani un jour après l'inoculation mais aucune différence dans les densités de pathogènes n'a été observée après trois jours entre le sol ayant reçu le bouillon de culture et le sol témoin. Les études de microcosmes ont révélé que la suspension cellulaire de RB14-C n'a également pu inhiber la croissance de R. solani sur les filtres enfouis dans le sol. Toutefois, un effet inhibiteur a été obtenu lorsque le bouillon de culture de la bactérie fut employé. L'impact de RB14-C sur la biomasse fongique fut également estimé en mesurant la concentration d'ergostérol dans le sol. Nous avons déterminé que l'ergostérol provenait principalement de R. solani, et il n'y avait pas la différence significative dans son contenu entre les sols traités ou non avec RB14-C. Les résultats indiquent que l'atténuation de Rhizoctonia par B. subtilis RB14-C n'est probablement pas reliée à la diminution de la population du pathogène dans le sol.Mots clés : B. subtilis, R. solani, ergostérol, microflore indigène.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: May 1, 2005

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
nrc/cjm/2005/00000051/00000005/art00005
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more