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Scanning electron microscopy analysis of aged Mycobacterium tuberculosis cells

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Abstract:

Most electron microscopy studies of Mycobacterium tuberculosis ultrastructure were performed in the 1950s and 1960s and lack high resolution by modern standards. This study was performed to re-evaluate the fine structure of M. tuberculosis using modern scanning electron microscopy. Bacteria were grown in rich medium with a constant supply of oxygen for several weeks. Results show that surface bleb-like structures accumulate as cultures age. The most unusual feature of aging M. tuberculosis cultures is that they develop extracellular fibrils, which could play roles of adhering cells to surfaces and to one another.Key words: Mycobacterium tuberculosis, SEM, stationary phase, surface blebs, extracellular fibrils.

La plupart des études faites à l'aide de la microscopie électronique sur l'ultrastructure du Mycobacterium tuberculosis ont été effectuées pendant les années 1950 et 1960 et elles manquent de haute résolution par rapport aux normes modernes. Cette étude a été effectuée afin de réévaluer la structure fine de M. tuberculosis en utilisant la microscopie électronique scanographique moderne. Les bacteria ont été cultivés dans un milieu riche et fournis continuellement de l'oxygène au cours de plusieurs semaines. Il s'avère que des structures superficielles qui ressemblent à des bulles accumulent au fur et à mesure que les cultures vieillissent. L'aspect le plus insolite des cultures M. tuberculosis vieillissantes, c'est qu'elles développent des fibrilles extracellulaires qui joueraient le rôle d'adhérer les cellules aux surfaces, ainsi que l'une à l'autre.Mots clés : Mycobacterium tuberculosis, MES, phase stationnaire, bulles superficielles, fibrilles extracellulaires.

Document Type: Research Article

Publication date: March 1, 2005

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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