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Functional characterization of KlHEM13, a hypoxic gene of Kluyveromyces lactis

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Abstract:

The KlHEM13 gene of Kluyveromyces lactis encoding the coproporphyrinogen oxidase (EC 1.3.3.3), an oxygen-requiring enzyme that catalyzes the sixth step of heme biosynthesis, was cloned and functionally characterized. The coding and upstream regions of KlHEM13 were analyzed and the putative cis regulatory elements were discussed in relation to the mechanisms of regulation of this hypoxic gene in K. lactis.Key words: coproporphyrinogen oxidase (CPO), HEM13, hypoxic genes, Kluyveromyces lactis.

Le gène KlHEM13 de Kluyveromyces lactis codant la coproporphyrinogène oxydase (EC 1.3.3.3), une enzyme nécessitant de l' oxygène et catalysant la sixième étape de la biosynthèse de l'hème, a été cloné et sa fonction caractérisée. La région codante et celle en aval de KlHEM13 sont analysées et des éléments régulateurs putatifs en cis sont discutés en lien avec les mécanismes de régulation de ce gène hypoxique chez K. lactis.Mots clés : coproporphyrinogène oxydase (CPO), HEM13, gènes hypoxiques, Kluyveromyces lactis.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: March 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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