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Novel antibacterial proteins from entomocidal crystals of Bacillus thuringiensis ssp. israelensis

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Abstract:

Proteins with molecular masses of 36 and 34 kDa (Bti36 and Bti34) were isolated from entomocidal crystals formed by Bacillus thuringiensis ssp. israelensis cells. The samples of Bti36 contained the admixture of a protein with a molecular mass of 33 kDa (Bti33), apparently a product of proteolysis of Bti36. These 3 proteins are significantly different in N-terminal sequences from known -endotoxins of B. thuringiensis and show antibacterial activity toward Micrococcus luteus. The combination of Bti36 and Bti33 also suppresses the growth of some other microorganisms including Streptomyces chrysomallus. The effects of the mixture of Bti36 and Bti33 on the M. luteus cell surface and on the surface of S. chrysomallus cells and exospores are similar, but they are different from the effect of endotoxin Cry11A on micrococcal cells.Key words: Bacillus thuringiensis, -endotoxins, antibacterial activity, Micrococcus luteus, Streptomyces chrysomallus.

Nous avons isolé des protéines de masse moléculaire de 36 et de 34 kDa (Bti36 et Bti34) à partir des cristaux entomocides formés par des cellules de Bacillus thuringiensis ssp. israelensis. Les échantillons de Bti36 renfermaient un sous-population de protéines de masse moléculaire de 33 kDa (Bti33), vraisemblablement un produit protéolytique de Bti36. Ces 3 protéines divergeaient significativement dans leurs séquences N-terminales de -endotoxines connues chez B. thuringiensis et démontraient une activité antibactérienne contre Micrococcus luteus. La combinaison de Bti36 et Bti33 a également inhibé la croissance d'autres micro-organismes dont Streptomyces chrysomallus. Les effets du mélange de Bti36 et Bti33 sur la surface cellulaire de M. luteus et sur la surface de cellules et d'exospores de S. chrysomallus étaient semblables, mais différaient de l'effet de l'endotoxine Cry11A sur des cellules micrococcales.Mots clés : Bacillus thuringiensis, -endotoxines, activité antibactérienne, Micrococcus luteus, Streptomyces chrysomallus.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2005-02-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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