Skip to main content

Biological control of collar rot disease with broad-spectrum antifungal bacteria associated with groundnut

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. niger, Rhizoctonia bataticola, Rhizoctonia solani, and Sclerotium rolfsii, and 3 biotrophic fungi, Cercospora arachidicola, Phaeoisariopsis personata, and Puccinia arachidis. Pseudomonas sp. GRS 175, Pseudomonas aeruginosa GPS 21, GSE 18, GSE 19, and GSE 30, and their cell-free culture filtrates were highly antagonistic to all the test fungi. The cell-free culture filtrates of these bacteria were fungicidal and induced mycelial deformations including hyphal bulging and vacuolization in necrotrophic fungi. The cell-free culture filtrates at 10% (v/v) concentration significantly inhibited the spore germination of biotrophic fungi. In the greenhouse, P. aeruginosa GSE 18 emerged as an effective biocontrol agent of collar rot closely followed by P. aeruginosa GSE 19. The bacterium applied as a seed treatment reduced the pre-emergence rotting and postemergence wilting by >60%. Pseudomonas aeruginosa GSE 18 effectively colonized the groundnut rhizosphere, both in native and in A. niger infested potting mixtures. Ninety-day-old peat formulation of P. aeruginosa GSE 18 had biocontrol ability comparable with the midlog-phase cells. Pseudomonas aeruginosa GSE 18, tolerant to thiram, in combination with the fungicide had an improved collar rot control. The present study was a successful attempt in selection of broad-spectrum and fungicide tolerant biocontrol agents that can be a useful component of integrated management of collar rot.Key words: Arachis, biocontrol, crown rot, peanut.

L'activité anti-fongique à large spectre de bactéries associées à 6 habitats de l'arachide, ainsi que leur potentiel de suppression de la rouille de l'arachide causée par Aspergillus niger ont été évalués. Trois cent quatre-vingt-trois souches ont été testées sur 8 pathogènes fongiques de l'arachide, incluant 5 champignons nécrotrophes : Aspergillus flavus, A. niger, Rhizoctonia bataticola, Rhizoctonia solani et Sclerotium rolfsii; et 3 champignons biotrophes : Cercospora arachidicola, Phaeoisariopsis personata et Puccinia arachidis. Les souches de Pseudomonas sp. GRS 175 et P. aeruginosa GPS 21, GSE 18, GSE 19 et GSE 30 ainsi que les filtrats acellulaires de culture (CAF) qui en sont issus ont été fortement antagonistes envers tous les champignons testés. Les CAF de ces bactéries ont été fongicides et ont causé des déformations du mycélium, incluant le renflement des hyphes et la vacuolisation chez les champignons nécrotrophes. Une concentration de 10 % (v/v) de CAF a inhibé significativement la germination des spores des champignons biotrophes. En serre, P. aeruginosa GSE 18 s'est révélé être un agent de lutte biologique efficace contre la rouille, suivi de près par P. aeruginosa GSE 19. Une application bactérienne pour traiter les graines a réduit l'émergence de la rouille et le flétrissement subséquent de plus de 60 %. Pseudomonas aeruginosa GSE 18 a colonisé efficacement la rhizosphère de l'arachide, tant en condition de base qu'en condition d'infestation par A. niger des mélanges d'empotage. Une formulation de tourbe âgée de 90 jours contenant P. aeruginosa possédait une activité de contrôle biologique comparable à celle de cellules en phase mi-logarithmique de croissance. Pseudomonas aeruginosa GSE 18 tolérante au thiram, combinée au fongicide, améliore le contrôle de la rouille. La présente étude a été un fructueux essai de sélection d'agents de lutte biologique à large spectre tolérants aux fongicides qui peuvent constituer des composantes utiles d'une lutte intégrée contre la rouille.Mots clés : Arachis, lutte biologique, rouille, arachide.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: February 1, 2005

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • Ingenta Connect is not responsible for the content or availability of external websites
nrc/cjm/2005/00000051/00000002/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more