Influence of carbon source and surface hydrophobicity on the aggregation of the yeast Kluyveromyces bulgaricus

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Abstract:

Aggregation of the yeast Kluyveromyces bulgaricus is mediated by the galactose-specific lectin KbCWL1. This lectin contains hydrophobic amino acids and its activity is calcium dependent. A specific fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid in the free acid form (ANS; Sigma Chemical Co., St. Louis, Missouri), was used to study the hydrophobic areas on the cellular surface of K. bulgaricus. Changes in surface hydrophobicity during the growth and aggregation of yeast cells were studied. Surface hydrophobicity increased during growth and depended on the amount of yeast cells in the culture medium. During growth, the size of the hydrophobic areas on the cell surface was measured using ANS and was found to increase with the percentage of flocculating yeasts. Our results strongly suggest that the hydrophobic areas of the cell walls of yeast cells are involved in the aggregation of K. bulgaricus.Key words: aggregation, carbon source, fluorescence probe, hydrophobicity, yeast.

L'agrégation de la levure Kluyveromyces bulgaricus est médiée par la lectine KbCWL1 spécifique du galactose KbCWL1. Cette lectine contient des acides aminés hydrophobes et son activité dépend du calcium. Une sonde fluorescente spécifique, acide 1-anilinonaphatalène-8-sulfonique sous forme d'acide libre, (ANS ; par Sigma Chemical Co., St. Louis, Missouri) a été utilisée afin d'étudier les régions hydrophobes sur la surface cellulaire de la levure. Les changements d'hydrophobicité de surface pendant la croissance et l'agrégation des levures ont été étudiés. L'hydrophobicité de surface augmente pendant la croissance et dépend de la quantité de levures dans le milieu de culture. Au cours de la croissance, la taille des zones hydrophobes sur la surface cellulaire a été mesurée avec l'ANS. Celle-ci augmente avec le pourcentage de levures floculantes. Nos résultats indiquent fortement que les régions hydrophobes de la paroi cellulaire des levures participent à l'agrégation des levures K. bulgaricus.Mots clés : agrégation, source de carbone, sonde fluorescente, hydrophobicité, levures.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2005

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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