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Characterization and regulation of new secondary metabolites from Aspergillus ochraceus M18 obtained by UV mutagenesis

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Abstract:

UV irradiation of Aspergillus ochraceus NRRL 3174 conidia led to stable mutations in ochratoxin and penicillic-acid pathways. These mutants, especially M18, produced an unexpectedly large number of new metabolites. Two new compounds were purified by TLC and HPLC and their chemical structures were determined. They are 2,10-dimethyl 4-hydroxy-6-oxo-4-undecen-7-yne (1) and 4-(3-methyl-2- butenyl) oxy 1-phenyl acetic acid (2). Compound 1 is very active against Gram-positive bacteria, such as Staphylococcus aureus and Bacillus subtilis, but inactive against Gram-negative bacteria, fungi, and yeasts. However, compound 2 has no antibiotic activity. The production of 1 was generally associated with growth, whereas that of compound 2 was dissociated from growth. The biosynthesis of these 2 metabolites was influenced by the sources of carbon and nitrogen.Key words: Aspergillus ochraceus, ochratoxin, penicillic acid, secondary metabolites, mycotoxins, regulation.

L'irradiation UV de conidies de Aspergillus ochraceus NRRL 3174 a mener à des mutations stables dans les voies de biosynthèse de l'ochratoxine et de l'acide pénicillique. Parmi ces mutants, le mutant M18 a produit une plusieurs de nouveaux métabolites. Deux nouveaux composés ont été purifiés par chromatographie TLC et HPLC et leurs structures chimiques ont été déterminées. Il s'agit du 2,10-diméthyl 4-hydroxy-6-oxo-4-undecen-7-yne (1) et de l'acide 4-(3-méthyl-2- butenyl) oxy 1-phényl acétique (2). Le composé 1 est très actif contre les bactéries Gram positives telles que Staphylococcus aureus et Bacillus subtilis mais est inactif contre les bactéries Gram négatives, les champignons et les levures. Le composé 2 ne présente aucune activité antibiotique. La production du composé 1 était généralement associée à la croissance alors que celle du composé 2 en était dissociée. La biosynthèse de ces 2 métabolites a été influencée par les sources de carbone et d'azote.Mots clés : Aspergillus ochraceus, ochratoxine, acide pénicillique, métabolites secondaires, mycotoxines, régulation.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2005-01-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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