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Antimicrobial susceptibility of lactic acid bacteria isolated from a cheese environment

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In the production of the Spanish traditional blue-veined Cabrales cheese, lactic acid bacteria strains free of antibiotic resistance that have a transferrable capacity are necessary as components of a specific starter. To select for these bacteria, the minimum inhibitory concentration (MIC) of 12 antibiotics and 2 mixtures (containing -lactamase inhibitor and penicillin) were determined by microbroth and agar dilution techniques in 146 strains belonging to the genera Lactococcus, Enterococcus, Lactobacillus, and Leuconostoc. The antibiotic-resistance profiles of Lactococcus and Enterococcus species were different from those of Lactobacillus and Leuconostoc, but clear genus- or species-associated patterns were not observed. Cefoxitin and metronidazole were not effective against bacteria of these genera. The MICs of -lactam antibiotics for lactobacilli and leuconostoc isolates were higher than those for lactococci and enterococci, but no strain was clinically resistant. All lactobacilli and leuconostoc isolates were resistant to high levels of vancomycin, a type of resistance not seen among the tested members of the genera Lactococcus and Enterococcus. The majority of the observed resistance appeared to be either intrinsic or nonspecific, although some strains of Lactococcus lactis, Enterococcus spp., and Lactobacillus spp. were resistant to antibiotics, such as chloramphenicol, erythromycin, clindamycin, or tetracycline.Key words: Lactococcus, Lactobacillus, Leuconostoc, lactic acid bacteria, antibiotic resistance, antimicrobials.

Afin de sélectionner des souches de bactéries lactiques dépourvues de résistance aux antibiotiques ayant une capacité de transfert en tant que de composantes d'un ferment spécifique de fromage traditionnel espagnol Carbales bleu veiné, la concentration minimale inhibitrice (CMI) de 12 antibiotiques et de 2 mélanges (un inhibiteur de la -lactamase et la pénicilline) a été déterminée par microdilution en milieu liquide et sur agar chez 146 souches appartenant aux genres Lactococcus, Enterococcus, Lactobacillus et Leuconostoc. Les profils de résistance aux antibiotiques des espèces de Lactococcus et d'Enterococcus différaient de ceux de Lactobacillus et de Leuconostoc, mais aucun profil associé au genre ou à l'espèce n'a été observé. La cefoxitine et le metronidazole furent inefficaces contre les bactéries de ces genres. Les CMI des antibiotiques -lactam chez les isolats de lactobacilles et de leuconostocs étaient supérieures à celles des lactocoques et des entérocoques, mais aucune souche n'était cliniquement résistante. Tous les isolats de lactobacilles et de leuconostocs étaient résistants à des niveaux élevés de vancomycine, un type de résistance qui n'a pas été rencontré chez les membres analysés des genres Lactococcus et Enterococcus. La majorité des résistances observées semblaient être soit intrinsèque ou non-spécifique, bien que certaines souches de Lactococcus lactis, Enterococcus spp. et Lactobacillus spp. étaient résistantes à des antibiotiques comme le chloramphénicol, l'érythromycine, la clindamycine ou la tétracycline.Mots clés : Lactococcus, Lactobacillus, Leuconostoc, bactéries lactiques, résistance aux antibiotiques, antimicrobiens.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2005-01-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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