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Phylogenetic analysis of bacterial populations in waters of the former Texcoco Lake, Mexico

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Abstract:

Molecular techniques were used to compare the compositions of the bacterial communities of the 2 following lagoons from the former soda Texcoco Lake, Mexico: the restored Facultativa lagoon and the Nabor Carrillo lagoon. Ribosomal intergenic spacer analysis (RISA) revealed that bacterial communities of the 2 lagoons were different and presented a relatively low diversity. Clone libraries of 16S rDNA genes were constructed, and significant phylotypes were distinguished by restriction fragment length polymorphism (RFLP). A representative clone from each phylotype was partially sequenced. Molecular identification and phylogenetic analyses based on ribosomal sequences revealed that the Facultativa lagoon harbored mainly - and -Proteobacteria, low G+C Gram-positive bacteria, and several members of the Halobacteriaceae family of archaea. The Nabor Carrillo lagoon mainly included typical halophilic and alkaliphilic low G+C Gram-positive bacteria, -Proteobacteria, and -Proteobacteria similar to those found in other soda lakes. Several probably noncultured new bacterial species were detected. Three strains were isolated from the Nabor Carrillo lagoon, their partial 16S rDNA sequences were obtained. On this basis, they were identified as Halomonas magadiensis (H1), Halomonas eurihalina (H2), and Staphylococcus sciuri (H3). This is the first study that uses molecular techniques to investigate potential genetic diversity in the Texcoco lakes. In this preliminary evaluation, we infer the presence of alkalophilic, halophilic, or haloalkaliphilic bacteria potentially useful for biotechnology.Key words: bacterial diversity, 16S rDNA gene, soda lakes, former soda Texcoco Lake, Mexico, alkaliphiles, halophiles, haloalkaliphiles.

Des techniques de biologie moléculaire ont été utilizées pour comparer la composition des communautés bactériennes dans 2 lagunes de l'ancien lac sodique de Texcoco au Mexique: la lagune Facultativa et la lagune Nabor Carrillo. L'analyse des espaces intergéniques ribosomiques (AEIR) révéla que les communautés bactériennes présentes dans les 2 lagunes sont différentes et relativement peu diverses. Des banques de clones du gène 16S rADN furent construites et les phylotypes significatifs séparés par analyse du polymorphisme de la taille de fragments de restriction (PTFR). Un clone représentatif de chaque phylotype fut partiellement séquencé. L'identification moléculaire et les analyses phylogénétiques basées sur les séquences ribosomales montrèrent que la lagune Facultativa contient principalement des proteobactéries gamma et beta, des bactéries Gram-positives faibles en G+C et plusieurs membres de la famille des Halobacteriaceae des archeae. La lagune Nabor Carrillo contenait principalement des bactéries Gram-positives halophiles et alcalophiles faible en G+C ainsi que des proteobactéries gamma et beta similaires a celles trouvées dans d'autres lacs sodiques. Plusieurs bactéries non cultivées et appartenant probablement à de nouvelles espèces furent aussi détectées. Trois souches furent isolées de la lagune Nabor Carrillo et la séquences de leur gènes 16S rADN fut obtenue permettant leur identification comme Halomonas magadiensis (H1), Halomonas eurihalina (H2) et Staphylococcus sciuri (H3). C'est la première étude utilizant des techniques moléculaires pour évaluer la diversité génétique potentielle de l'ancien lac Texcoco. Au cours de cette première évaluation, la présence de bactéries alcalophiles, halophiles et haloalcalophiles potentiellement intéressantes a été mise en évidence.Mots clés : diversité bactérienne, gène 16S rADN, lacs sodiques, ancien lac sodique Texcoco, Mexique, alcalophiles, halophiles, haloalcalophiles.

Document Type: Research Article

Publication date: December 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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