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Optimization of extracellular fungal peroxidase production by 2 Coprinus species

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Optimum culture conditions for the batch production of extracellular peroxidase by Coprinus cinereus UAMH 4103 and Coprinus sp. UAMH 10067 were explored using 2 statistical experimental designs, including 2-level, 7-factor fractional factorial design and 2-factor central composite design. Of the 7 factors examined in the screening study, the concentrations of carbon (glucose) and nitrogen (peptone or casitone) sources showed significant effects on the peroxidase production by Coprinus sp. UAMH 10067. The optimum glucose and peptone concentrations were determined as 2.7% and 0.8% for Coprinus sp. UAMH 10067, and 2.9% and 1.4% for C. cinereus UAMH 4103, respectively. Under the optimized culture condition the maximum peroxidase activity achieved in this study was 34.5 U·mL–1 for Coprinus sp. UAMH 10067 and 68.0 U·mL–1 for C. cinereus UAMH 4103, more than 2-fold higher than the results of previous studies.Key words: Coprinus peroxidase, central composite design, fractional factorial design, production optimization, response surface.

Nous avons étudié les conditions optimales de culture pour la production en lots de la peroxydase extracellulaire par Coprinus cinereus UAMH 4103 et Coprinus sp. UAMH 10067 à l'aide de 2 les approches expérimentales statistiques incluant un plan expérimental factoriel fractionnel à 2 niveaux et 7 facteurs et un plan expérimental composite central à 2 facteurs. Parmi les 7 facteurs examinés dans l'étude de criblage, les concentrations des sources de carbone (glucose) et d'azote (peptone et casitone) ont eu un impact significatif sur la production de la peroxydase par Coprinus sp. UAMH 10067. Il fut déterminé que les concentrations optimales de glucose et de peptone étaient respectivement de 2,7 % et de 0,8 % pour Coprinus sp. UAMH 10067 et de 2,9 % et 1,4 % pour C. cinereus UAMH 4103. En utilisant les conditions optimales de culture, l'activité peroxydase maximale atteinte dans cette étude fut de 34,5 U·mL–1 pour Coprinus sp. UAMH 10067 et de 68,0 U·mL–1 pour C. cinereus UAMH 4103, plus de 2 fois supérieure aux résultats d'études antérieures.Mots clés : peroxydase de Coprinus, plan expérimental central composite, plan expérimental fractionnel factoriel, optimisation de la production, surface de réponse.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2004-12-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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