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Genetic characterization of high-level gentamicin-resistant strains of Enterococcus faecalis in Iran

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The prevalence of resistance to high levels of gentamicin among 182 isolates of Enterococcus faecalis from 2 Iranian hospitals was 42%. Gentamicin resistance was associated with conjugative plasmids (>70 kb) in most strains. Fingerprinting using EcoRI and HindIII showed genetic variation among these plasmids and gave evidence of nosocomial outbreaks and persistence of infection in different wards of the study hospitals, as well as transfer of plasmids between genetically diverse isolates. Using EcoRI, hospital-based specific plasmid fingerprints were detected for the isolates that had previously proved to be unrelated by multilocus enzyme electrophoresis, suggesting the persistence of related plasmids at each hospital, though minor changes in these related plasmids could be detected with HindIII.Key words: Enterococcus faecalis, HLGR, plasmid profiling, plasmid fingerprinting.

La prévalence de la résistance à des hauts taux de gentamycine (RHTG) parmi les isolats de Enterococcus faecalis de deux hôpitaux d'Iran était de 42 %. La résistance à la gentamycine était associée avec des plasmides conjugatifs (>70 kb) dans la plupart des souches. La prise d'empreintes génétiques à l'aide de EcoRI et HindIII a révélé une variation génétique parmi ces plasmides, et a signalé la présence de contaminations nosocomiales et de la persistance d'infections à entérococques RHTG dans différents blocs des hôpitaux étudiés, de même qu'un transfert des plasmides entre des isolats génétiquement distincts. À l'aide de EcoRI, des empreintes de plasmides spécifiques ont été détectées chez des isolats hospitaliers qui avaient été auparavant considérés non apparentés par électrophorèse d'enzymes multilocus, ce qui dénote une persistance de plasmides apparentés dans chacun des hôpitaux, bien que des changements mineurs dans ces plasmides apparentés ont pu être détectés par HindIII.Mots clés : Enterococcus faecalis, RHTG, profils de plasmides, prise d'empreintes de plasmides.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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