Optimization of casein-based semisynthetic medium for growing of toxigenic Corinebacterium diphtheriae in a fermenter

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Diphtheria toxin is produced by growing Corinebacterium diphtheriae either in a semisynthetic casein-based medium or in the Pope–Lingood meat extract based medium. The World Health Organization advises the use of the semisynthetic one, as it has important advantages. Data on the composition of casein-based media and their ability to support high toxin production are not freely available. Important factors affecting toxin production during C. diphtheriae cultivation are the pH of the culture medium and the concentration of casein hydrolysate and of Fe2+. We established that the optimal pH for toxin production is 7.2. The highest yield of toxin was obtained using a casein hydrolysate concentration of 35.0 g/L and a Fe2+ concentration of 0.05–0.41 µg/mL. Under these conditions, diphtheria toxin with higher purity and yield compared with the batches obtained using the meat-based medium of Pope–Lingood was produced.Key words: diphtheria toxin, diphtheria toxoid, cultivation, semisynthetic medium.

La toxine diphtérique est produite à partir d'une culture de Corinebacterium diphtheriae soit dans un milieu semi-synthétique à base de caséine, soit dans le milieu à base d'extrait de viande Pop–Lingood. L'Organisation Mondiale de la Santé encourage l'utilisation du milieu semi-synthétique puisqu'il comporte d'importants avantages. Les données sur la composition des milieux à base de caséine et de leur capacité à soutenir une production élevée de toxine ne sont pas facilement disponibles. Les principaux facteurs influençant la production de la toxine au cours de la culture de C. diphtheriae sont le pH du milieu de culture, la concentration de l'hydrolysat de caséine, et les ions Fe2+. Nous avons déterminé que le pH optimal pour la production de la toxine était de 7,2. Le rendement maximal de toxine fut obtenu avec une concentration d'hydrolysat de caséine de 35,0 g/L et de Fe2+ de 0,05 – 0,41 µg/mL. Dans ces conditions, le rendement et la pureté de la toxine diphtérique était supérieure à celle de lots obtenus à partir de cultures dans un milieu à base d'extrait de viande Pop–Lingood.Mots clés : toxine diphtérique, anatoxine diphtérique, culture, milieu semi-synthétique.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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