Characterization of Paenibacillus popilliae rRNA operons

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The terminal 39 nucleotides on the 3' end of the 16S rRNA gene, along with the complete DNA sequences of the 5S rRNA, 23S rRNA, tRNAIle, and tRNAAla genes were determined for Paenibacillus popilliae using strains NRRL B-2309 and Dutky 1. Southern hybridization analysis with a 16S rDNA hybridization probe and restriction-digested genomic DNA demonstrated 8 copies of the 16S rRNA gene in P. popilliae strains KLN 3 and Dutky 1. Additionally, the 23S rRNA gene in P. popilliae strains NRRL B-2309, KLN 3, and Dutky 1 was shown by I-CeuI digestion and pulsed-field gel electrophoresis of genomic DNA to occur as 8 copies. It was concluded that these 3 P. popilliae strains contained 8 rrn operons. The 8 operon copies were preferentially located on approximately one-half of the chromosome and were organized into 3 different patterns of genes, as follows: 16S-23S-5S, 16S-ala-23S-5S, and 16S-5S-ile-ala-23S-5S. This is the first report to identify a 5S rRNA gene between the 16S and 23S rRNA genes of a bacterial rrn operon. Comparative analysis of the nucleotides on the 3' end of the 16S rRNA gene suggests that translation of P. popilliae mRNA may occur in Bacillus subtilis and Escherichia coli.Key words: Paenibacillus, milky disease, rrn operon, rRNA, tRNA.

Les 39 nucléotides terminaux de la région 3' du gène de l'ARNr 16S, ainsi que les séquences entières d'ADN des gènes de l'ARNr 5S, l'ARNr 23S, l'ARNtIle, et l'ARNtAla ont été déterminés chez Paenibacillus popilliae en utilisant les souches NRRL B-2309 et Dutky 1. Une analyse par hybridation de type Southern, à l'aide d'une sonde d'hybridation de l'ADNr 16S et de l'ADN génomique digéré par restriction, a révélé 8 copies du gène de l'ARNr 16S chez les souches de P. popilliae KLN 3 et Dutky 1. De plus, il fut démontré par digestion avec I-CeuI et électrophorèse sur gel en champ pulsé que le gène de l'ARNr 23S chez les souches de P. popilliae NRRL B-2309, KLN 3 et Dutky 1 était présent dans l'ADN génomique en 8 copies. Nous avons déduit que ces trois souches de P. popilliae renfermaient 8 opérons rrn. Les 8 copies des opérons était localisées de préférence sur environ la moitié du chromosome et étaient organisées en trois motifs différents de gènes: 16S-23S-5S, 16S-ala-23S-5S, et 16S-5S-ile-ala-23S-5S. Ceci représente le premier cas identifiant un gène de l'ARNr 5S entre les gènes d'ARNr 16S et 23S dans un opéron bactérien rrn. Une analyse comparative des nucléotides de la terminaison 3' du gène de l'ARNr 16S indique que la traduction de l'ARNm de P. popilliae pourrait s'accomplir chez Bacillus subtilis et Escherichia coli.Mots clés : Paenibacillus, maladie laiteuse, opéron rrn, ARNr, ARNt.

Document Type: Research Article

Publication date: October 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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