Influence of carbon and nitrogen sources on growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus

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Abstract:

The effects of different carbon and nitrogen sources on the growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus were investigated. The amino acids asparagine, aspartic acid, and glutamic acid affected microbial growth and nitrogenase activity. Several enzymatic activities involved in the tricarboxylic acid cycle were affected by the carbon source used. In addition, glucose and gluconate significantly increased the oxygen consumption (respiration rate) of whole cells of G. diazotrophicus grown under aerobic conditions. Enzymes responsible for direct oxidation of glucose and gluconate were especially active in cells grown with sucrose and gluconate. The presence of amino acids in the apoplastic and symplastic sap of sugarcane stems suggests that these compounds might be of importance in the regulation of growth and nitrogenase activity during the symbiotic association. The information obtained from the plant–bacterium association together with the results of other biochemical studies could contribute to the development of biotechnological applications of G. diazotrophicus.Key words: Gluconacetobacter diazotrophicus, endophyte, metabolism, nitrogen fixation.

Le travail consiste à étudier l'effet des différentes sources de carbone et d'azote sur la croissance, l'activité nitrogenase et la metabolisme du carbone sur les cellules de Gluconacetobacter diazotrophicus en milieu de culture. Les acides aminés, asparagine, l'acide aspartique et l'acide glutamique ont affecté la croissance microbienne et la fixation d'azote (l'activité nitrogenase). Aussi, plusieurs activités enzymatiques impliquées dans le cycle des acides tricarboxyliques ont été affectées par la source carbonique utilisée. De plus, le glucose et le gluconate ont significativement augmenté la consommation d'oxygène (le taux respiratoire) des cellules entieres de G. diazotrophicus cultivé dans des conditions aérobics. Les enzymes responsables de l'oxydation directe du glucose et du gluconate étaient spécialement actifs dans les cellules en croissance avec le saccharose et le gluconate. La présence d'acides aminés dans l'apoplaste et le jus de symplaste de tiges de canne à sucre suggèrent que ces composés puissent avoir de l'importance dans le règlement de la croissance et de l'activité nitrogenase pendant l'association symbiotique. L'information obtenue de l'association plantes–bactéries ensemble avec d'autres études biochimiques pourrait contribuer pour trouver les demandes biotechnologiques de Gluconacetobacter diazotrophicus.Mots clés : Gluconacetobacter diazotrophicus, endophyte, métabolisme, fixation d'azote.

Document Type: Research Article

Publication date: September 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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