Human IgA inhibits adherence of Acanthamoeba polyphaga to epithelial cells and contact lenses

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Abstract:

Specific anti-Acanthamoeba IgA antibodies have been detected in the serum and tears of patients and healthy individuals. However, the role of human secretory IgA antibodies in inhibiting the adherence of Acanthamoeba had not been previously investigated. Therefore, the purpose of this study was to purify secretory IgA from human colostrum and analyze its effect on the adherence of Acanthamoeba trophozoites to contact lenses and Madin–Darby canine kidney (MDCK) cells. IgA antibodies to Acanthamoeba polyphaga in colostrum of healthy women as well as in saliva and serum of healthy subjects were analyzed by ELISA and Western blot analysis. In serum, saliva, and colostrum, we detected IgA antibodies that recognized several antigens of A. polyphaga. In addition, colostrum and IgA antibodies purified from it inhibited adherence of A. polyphaga trophozoites to contact lenses and MDCK cells. These results suggest that IgA antibodies may participate in the resistance to the amoebic infection, probably by inhibiting the adherence of the trophozoites to contact lenses and corneal epithelial cells.Key words: Acanthamoeba polyphaga, free-living amoebas, colostrum, IgA.

Des anticorps IgA spécifiques à Acanthamoeba ont été détectés dans le sérum et les larmes de patients et d'individus sains. Toutefois, le rôle des anticorps IgA humains sécrétoires dans l'inhibition de l'adhérence de Acanthamoeba n'a jamais été étudié. Le but de cette étude fut donc de purifier les IgA sécrétoires du colostrum humain et d'analyser leur effet sur l'adhérence des trophozoïte de Acanthamoeba à des lentilles cornéennes et à des cellules canines de rein Mardin–Darby (MDCK). Nous avons analysé par ELISA et par immunobuvardage de type Western les anticorps IgA dirigés contre A. polyphaga dans le colostrum de femmes saines de même que dans la salive et dans le sérum de sujets sains. Nous avons détecté dans le sérum, la salive et le colostrum des anticorps IgA reconnaissant plusieurs antigènes de A. polyphaga. De plus, le colostrum et les anticorps IgA purifiés à partir de celui-ci ont inhibé l'adhérence de trophozoïtes de A. polyphaga aux lentilles cornéennes et aux cellules MDCK. Ces résultats indiquent que les anticorps IgA pourraient participer à la résistance à l'infection amibienne, probablement en inhibant l'adhérence des trophozoïtes aux lentilles et aux cellules épithéliales cornéennes.Mots clés : Acanthamoeba polyphaga, amobes à l'état libre, colostrum, IgA.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: September 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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