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Sporocarps of Pisolithus albus as an ecological niche for fluorescent pseudomonads involved in Acacia mangium Wild – Pisolithus albus ectomycorrhizal symbiosis

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Fresh sporocarps and root and soil samples were collected under a monospecific forest plantation of Acacia mangium in Dagana in Northern Senegal and checked for the presence of fluorescent pseudomonads. No bacteria were detected except from sporocarps collected with adhering soil and hyphal strands. Pisolithus sporocarps were dried at 30 °C for 2 weeks, ground, passed through a 2-mm sieve and mixed together. This dry sporocarp powder (DSP) was used to inoculate and form mycorrhizas on A. mangium seedlings in a glasshouse experiment. After 3 months culture, plant growth was increased in the DSP treatment but no ectomycorrhizas were present on the A. mangium root systems; however fluorescent pseudomonads were recorded in the cultural soil. The stimulatory effects on the plant growth were maintained for 6 months. However, fluorescent pseudomonads were no longer detected and 35% of the short roots were ectomycorrhizal. Some of the fluorescent pseudomonad isolates detected after 3 months stimulated the radial fungal growth in axenic conditions. These observations suggest that these bacteria are closely associated with the Pisolithus fructifications and could interact with the ectomycorrhizal symbiosis establishment.Key words: ectomycorrhizal symbiosis, fluorescent pseudomonads, Pisolithus, Acacia.

Des sporocarpes frais, ainsi que des échantillons de racines et de sol ont été recueillis dans une plantation forestière monospécifique d'Acacia mangium à Dagana dans le nord du Sénégal, et caractérisés quant à la présence de pseudomonades fluorescentes. Aucune bactérie ne fut détectée sauf chez les sporocarpes recueillis avec du sol adhérent et des hyphes. Des sporocarpes de Pisolithus ont été séchés à 30 °C pendant deux semaines, broyés, filtrés à travers un tamis de 2 mm et mélangés. Cette poudre sèche de sporocarpes (PSS) a été utilisée pour l'inoculation et la formation de mycorrhizes dans des semis de A. mangium lors d'une expérience en serre. Après trois mois de culture, la croissance des plantes a augmenté suite au traitement au PSS, mais aucune ectomycorrhize n'était présente dans le système racinaire de A. mangium; cependant, des speudomonades fluorescentes ont été répertoriées dans le sol de culture. Les effets stimulateurs observés sur la croissance des plants se sont maintenus pendant six mois. Cependant, les pseudomonades fluorescentes n'étaient alors plus détectables tandis que 35 % des racines courtes étaient ectomycorrhiziennes. Quelques uns isolats de pseudomonades fluorescentes détectés après 6 mois ont stimulé la croissance radiale fongique dans des conditions axéniques. Ces observations suggèrent que ces bactéries soient intimement associées à la fructification de Pisolithus et qu'elles puissent interagir avec l'établissement d'une symbiose ectomycorrhizienne.Mots clés : symbiose ectomycorrhizienne, pseudomonades fluorescentes, Pisolithus, Acacia.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: September 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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