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Isolation and transcriptional regulation of the Kluyveromyces lactis FBA1 (fructose-1,6-bisphosphate aldolase) gene

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Abstract:

Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is mediated by heme since expression was higher in a mutant defective in heme biosynthesis. KlFBA1 transcription is not induced by calcium-shortage, low temperature, or at stationary phase. These data suggest that KlFBA1 plays a role in the balance between oxidative and fermentative metabolism and that this gene is differentially regulated in K. lactis and Saccharomyces cerevisiae, i.e., a respiratory vs. fermentative yeast.Key words: FBA1, fructose-1,6-bisphosphate aldolase, Kluyveromyces, transcriptional regulation, yeast.

Nous faisons la description du clonage et de la régulation transcriptionnelle du gène KlFBA1, qui code une fructose-1,6-bisphosphate aldolase de classe II de la levure Kluyveromyces lactis. L'ARNm de KlFBA1 diminue de façon transitoire lors du passage de conditions hypoxiques à totalement aérobies et augmente lors du passage inverse. Cette régulation est rendue possible par l'hème puisque l'expression était supérieure chez un mutant déficient dans la biosynthèse de l'hème. La transcription de la KlFBA1 n'est pas induite par une carence en calcium, des températures basses ou la phase stationnaire. Ces données indiquent que KlFBA1 joue un rôle dans l'équilibre entre le métabolisme oxydatif et fermentatif, et que ce gène est régulé de façon différentielle chez K. lactis et Saccharomyces cerevisiae, c'est-à-dire une levure respiratoire versus fermentative.Mots clés : FBA1, fructose-1,6-bisphosphate aldolase, Kluyveromyces, régulation transcriptionnelle, levure.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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