Production of ribonucleotides by autolysis of Pichia anomala mutant and some physiological activities

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Various mutants of Pichia anomala were isolated by ethyl methanesulfonate (EMS) treatment and UV irradiation through cycloheximide resistance and KCl sensitivity. The selected mutant HA-2 accumulated a higher content of RNA and grew faster than the wild-type strain in yeast extract–malt (YM) broth. Autolysis of the HA-2 mutant at 60 °C and pH 7.0 for 6 h was the best condition to obtain maximum yields of 5-ribonucleotides, inosinic monophosphate (IMP) (6.2 mg/g biomass) and guanylic monophosphate (GMP) (35.5 mg/g biomass). The yield of adenylic monophosphate (AMP) (7.8 mg/g biomass) was optimal at 60 °C at pH 6.5 for 6 h. The inhibitory activity of the angiotensin-converting enzyme and the nitrite-scavenging activity for autolysates of the HA-2 mutant were about 13.0% and 47.0% higher than those of native strain, respectively.Key words: Pichia anomala mutant, 5-ribonucleotides, adenylic monophosphate (AMP), guanylic monophosphate (GMP), inosinic monophosphate (IMP), autolysate.

Divers mutants de Pichia anomala ont été isolés par le traitement au methanesulfonate d'éthyle (EMS) et à l'irradiation aux UV grâce à la résistance au cycloheximide et à la sensibilité au KCl. Le mutant selectionné HA-2 accumulait un plus haut contenu d'ARN et sa croissance était plus rapide que la souche sauvage dans le milieu YM. L'autolyse du mutant HA-2 à 60 °C, pH 7.0 durant 6 h a été retenue pour maximiser les rendements de 5-ribonucleotides, d'IMP (6.2 mg/g biomasse) et de GMP (35.5 mg/g biomasse). Le rendement d'AMP (7.8 mg/g biomasse) était optimal pour 6 h d'incubation à 60 °C, pH 6.5. L'activité inhibitrice de la conversion enzymatique de l'angiotensine et l'activité d'enlèvement du nitrite dans les autolysats du mutant HA-2 étaient respectivement de 13.0 % et 47.0 % plus élevées que ceux de la souche sauvage.Mots clés : Pichia anomala mutant, 5-ribonucleotides, adenylic monophosphate (AMP), guanylic monophosphate (GMP), inosinic monophosphate (IMP), autolysate.

Document Type: Research Article

Publication date: July 1, 2004

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more