Production of ribonucleotides by autolysis of Pichia anomala mutant and some physiological activities

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Various mutants of Pichia anomala were isolated by ethyl methanesulfonate (EMS) treatment and UV irradiation through cycloheximide resistance and KCl sensitivity. The selected mutant HA-2 accumulated a higher content of RNA and grew faster than the wild-type strain in yeast extract–malt (YM) broth. Autolysis of the HA-2 mutant at 60 °C and pH 7.0 for 6 h was the best condition to obtain maximum yields of 5-ribonucleotides, inosinic monophosphate (IMP) (6.2 mg/g biomass) and guanylic monophosphate (GMP) (35.5 mg/g biomass). The yield of adenylic monophosphate (AMP) (7.8 mg/g biomass) was optimal at 60 °C at pH 6.5 for 6 h. The inhibitory activity of the angiotensin-converting enzyme and the nitrite-scavenging activity for autolysates of the HA-2 mutant were about 13.0% and 47.0% higher than those of native strain, respectively.Key words: Pichia anomala mutant, 5-ribonucleotides, adenylic monophosphate (AMP), guanylic monophosphate (GMP), inosinic monophosphate (IMP), autolysate.

Divers mutants de Pichia anomala ont été isolés par le traitement au methanesulfonate d'éthyle (EMS) et à l'irradiation aux UV grâce à la résistance au cycloheximide et à la sensibilité au KCl. Le mutant selectionné HA-2 accumulait un plus haut contenu d'ARN et sa croissance était plus rapide que la souche sauvage dans le milieu YM. L'autolyse du mutant HA-2 à 60 °C, pH 7.0 durant 6 h a été retenue pour maximiser les rendements de 5-ribonucleotides, d'IMP (6.2 mg/g biomasse) et de GMP (35.5 mg/g biomasse). Le rendement d'AMP (7.8 mg/g biomasse) était optimal pour 6 h d'incubation à 60 °C, pH 6.5. L'activité inhibitrice de la conversion enzymatique de l'angiotensine et l'activité d'enlèvement du nitrite dans les autolysats du mutant HA-2 étaient respectivement de 13.0 % et 47.0 % plus élevées que ceux de la souche sauvage.Mots clés : Pichia anomala mutant, 5-ribonucleotides, adenylic monophosphate (AMP), guanylic monophosphate (GMP), inosinic monophosphate (IMP), autolysate.

Document Type: Research Article

Publication date: July 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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