Denitrifying bacteria in bulk and maize-rhizospheric soil: diversity and N2O-reducing abilities

Authors: Chèneby, D; Perrez, S; Devroe, C; Hallet, S; Couton, Y; Bizouard, F; Iuretig, G; Germon, J C; Philippot, L

Source: Canadian Journal of Microbiology, Volume 50, Number 7, July 2004 , pp. 469-474(6)

Publisher: NRC Research Press

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Abstract:

The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of these isolates were identified as denitrifiers based on their ability to reduce nitrate to N2O or N2. 16S rDNA fragments amplified from the denitrifying isolates were analysed by restriction fragment length polymorphism. Isolates were grouped according to their restriction patterns, and 16S rDNA of representatives from each group were sequenced. A plant dependent enrichment of Agrobacterium-related denitrifiers has been observed resulting in a modification of the structure of the denitrifying community between planted and bulk soil. In addition, the predominant isolates in the rhizosphere soil were not able to reduce N2O while dominant isolates in the bulk soil evolve N2 as a denitrification product.Key words: denitrification, diversity, rhizosphere, N2O, maize.

L'objectif de ce travail était de déterminer l'effet de la rhizosphère du maïs sur la diversité des bactéries dénitrifiantes. Pour cela, nous avons obtenu une collection d'isolats à partir d'un sol nu et d'un sol planté avec du maïs. Nous avons ainsi isolé 3240 bactéries nitrate réductrices et 188 ont été identifiées comme dénitrifiante sur la base d'une réduction du nitrate en N2O ou N2. Les amplifiats du 16S rDNA des bactéries dénitrifiantes isolées ont été analysés par RFLP. Les isolats ont ensuite été regroupés en fonction du profil de restriction obtenu et l'ADNr 16S d'isolats de chacun des groupes obtenus a été séquencé. Nous avons ainsi observé une sélection des dénitrifiants proche du genre Agrobacterium dans la rhizosphere du maïs entraînant une modification de la structure de la communauté dénitrifiante entre le sol planté et le sol nu. De plus, les isolats dominants dans la rhizosphere ne sont pas capable de réduire le N2O alors que ceux dominants dans le sol nu produisent N2 comme produit terminal de la dénitrification.Mots clés : dénitrification, diversité, rhizosphère, N2O, maïs.

Document Type: Research Article

Publication date: July 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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