Enhancement of the antifungal activity of Bacillus subtilis F29-3 by the chitinase encoded by Bacillus circulans chiA gene

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Abstract:

Bacillus subtilis F29–3 is an antagonistic bacterium against a wide range of fungal species. In order to determine the effect of chitinase on the antifungal activity of B. subtilis F29–3, a 2.4-kb DNA fragment containing the chiA gene of Bacillus circulans WL-12 was ligated into a shuttle vector pHY300PLK and transformed into B. subtilis F29–3. A bioassay conducted on the culture supernatant showed that, in comparison to the B. subtilis control strain, B. subtilis F29–3 expressing the chiA gene exhibited a greater inhibition of spore germination of Botrytis elliptica, indicating that chitinase could enhance the antifungal function conferred by B. subtilis F29–3.Key words: Bacillus subtilis, antifungal activity, chiA, Bacillus circulans.

Bacillus subtilis F29–3 est un bactérie ayant une action antagoniste contre une large gamme d'espèces de champignons. Afin de déterminer l'impact de la chitinase sur l'activité antifongique de Bacillus subtilis F29–3, un fragment d'ADN de 2,4 kb renfermant le gène chiA de Bacillus circulans WL-12 a été ligué dans le vecteur navette pHY300PLK et transformé dans B. subtilis F29–3. Un bioessai effectué sur le surnageant de culture a démontré que, comparativement à la souche témoin de B. subtilis, B. subtilis F29–3 exprimant le gène chiA inhibe davantage la germination des spores de Botrytis elliptica, ce qui indique que la chitinase peut augmenter l'action antifongique de B. subtilis F29–3.Mots clés : Bacillus subtilis, activité antifongique, chiA, Bacillus circulans.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: June 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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