Inhibition of Pseudomonas aeruginosa adhesion to fibronectin by PA-IL and monosaccharides: involvement of a lectin-like process

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Pseudomonas aeruginosa adherence to fibronectin has been shown to be important to bacterial colonization and infection. To better understand the mechanisms involved in this interaction, the role of the carbohydrate moiety of the fibronectin molecule in P. aeruginosa adhesion was studied. Strain NK 125 502 adhered to immobilized fibronectin with an adherence index of 4.8 × 105 CFU/µg. Periodic oxidation of fibronectin markedly reduced the adhesion of P. aeruginosa, while a neuraminidase treatment increased bacteria adhesion. N-Acetylgalactosamine, N-acetylglucosamine, sialic acid, and also lectin PA-IL worked as efficient inhibitors in adhesion assays: 59%, 70.7%, 100%, and 60% of inhibition, respectively. We have demonstrated here the involvement of a lectin-like process in the interaction of P. aeruginosa NK 125 502 with immobilized fibronectin.Key words: Pseudomonas aeruginosa, fibronectin, adherence, lectins.

L'adhérence de Pseudomonas aeruginosa à la fibronectine est une des étapes primordiales pour le processus de colonisation et d'infection d'un tissue épithélial par ce micro-organisme. Afin de mieux comprendre les mécanismes mis en jeu, nous sommes intéressés aux glycosylations de la fibronectine et à leur rôle dans le processus d'adhésion de P. aeruginosa. La souche NK 125 502 adhère à la fibronectine immobilisée avec un indice de 4,8 × 105 UFC/µg. Une oxydation périodique de la fibronectine réduit l'adhérence de P. aeruginosa de façon très importante, alors qu'un traitement par la neuraminidase a augmenté l'adhérence des bactéries. La N-acetylgalactosamine, la N-acetylglucosamine, l'acide sialique ainsi que la lectine PA-IL de P. aeruginosa sont de forts compétiteurs d'adhérence (respectivement, 59 %, 70,7 %, 100 % et 60 % d'inhibition d'adhérence). Nous avons démontré la participation d'interactions de type lectine dans le processus d'adhésion de P. aeruginosa NK 125 502 à la fibronectine immobilisée.Mots clés : Pseudomonas aeruginosa, fibronectine, adhérence, lectines.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: May 1, 2004

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more