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Bacterial formyl peptides affect the innate cellular antimicrobial responses of larval Galleria mellonella (Insecta: Lepidoptera)

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The non-self cellular (hemocytic) responses of Galleria mellonella larvae, including the attachment to slides and the removal of the bacteria Xenorhabdus nematophila and Bacillus subtilis from the hemolymph, were affected by N-formyl peptides. Both N-formyl methionyl-leucyl-phenylalanine (fMLF) and the ester derivative decreased hemocyte adhesion in vitro, and both elevated hemocyte counts and suppressed the removal of both X. nematophila and B. subtilis from the hemolymph in vivo. The amide derivative and the antagonist tertiary-butoxy-carbonyl-methionyl-leucyl-phenylalanine (tBOC) increased hemocyte attachment to glass. The fMLF suppressed protein discharge from monolayers of granular cells with and without bacterial stimulation, while tBOC stimulated protein discharge. The peptide tBOC offset the effects of fMLF in vitro and in vivo. This is the first report implying the existence of formyl peptide receptors on insect hemocytes in which the compounds fMLF and tBOC inhibited and activated hemocyte activity, respectively.Key words: formyl peptides, hemocytes, Xenorhabdus, Bacillus.

Les réponses cellulaires (hémocytiques) au non-soi de la larve de Galleria mellonella, dont l'attachement initial aux lames et l'élimination de la bactérie Xenorhabdus nematophila et Bacillus subtilis de l'hémolymphe, ont été influencées par des peptides N-formylées. Le N-formyl methionyl-leucyl-phénylalanine (fMLF) et le dérivé ester ont tous deux diminué l'adhésion des hémocytes in vitro, et ont augmenté les comptes d'hémocytes tout en supprimant l'élimination in vivo de X. nematophila et de B. subtilis dans l'hémolymphe. Le dérivé amide et l'antagoniste, le butoxy-carbonyl-methionyl-leucyl-pénylalanine tertiaire (tBOC), ont augmenté l'adhérence des hémocytes au verre. Le fMLF a supprimé le relargage de protéines à partir de monocouches de cellules granulaires, avec ou sans stimulation bactérienne, alors que le tBOC a stimulé le relargage de protéines. Le peptide tBOC a neutralizé l'effet du fMLF in vitro et in vivo. Ceci est la première étude signalant l'existence de récepteurs à peptides formylés sur des hémocytes d'insecte, chez qui les composés fMLF et tBOC ont respectivement inhibé et stimulé l'activité.Mots clés : peptides formylés, hémocytes, Xenorhabdus, Bacillus.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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