Extracellular peroxidase production by Coprinus species from urea-treated soil

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Abstract:

Thirteen strains of inky-cap mushroom Coprinus species were evaluated for the production of extracellular peroxidase. The liquid fermentation was carried out in shake flasks containing 1% glucose, 0.5% peptone, 0.3% yeast extract, and 0.3% malt extract broth at 25 °C. Peroxidase activity was detected in the liquid culture of several Coprinus species, including C. echinosporus NBRC 30630; C. macrocephalus NBRC 30117; Coprinus spp. UAMH 10065, UAMH 10066, UAMH 10067, and 074, after 10 days of growth. Peroxidase production by Coprinus sp. UAMH 10067, a Coprinus species isolated from urea-treated soil, was comparable to that of C. cinereus and reached 15 U·mL–1 after 10 days. In addition, the peroxidase from Coprinus sp. UAMH 10067 was apparently more thermally stable than the enzyme produced by C. cinereus.Key words: Coprinus species, urea treatment, phenol, wastewater treatment.

La production de peroxydases extracellulaires par treize souches de champignons au chapeau noir d'encre de l'espèce Coprinus a été évaluée. La fermentation liquide a été accomplie à 25 °C dans des flacons agitateurs contenant un bouillon composé de 1 % de glucose, 0,5 % de peptone, 0,3 % d'extrait de levure et 0,3 % d'extrait de malt. L'activité peroxydase a été détectée dans la culture liquide de plusieurs espèces de Coprinus, notamment C. echinosporus NBRC 30630, C. macrocephalus NBRC 30117, Coprinus spp. UAMH 10065, UAMH 10066, UAMH 10067 et 074 après 10 jours de culture. La production de peroxydase par Coprinus sp. UAMH 10067, une espèce de Coprinus isolée d'un sol traité à l'urée, était comparable à celle de C. cinereus et a atteint 15 U·mL–1 après 10 jours. De plus, la peroxydase de Coprinus sp. UAMH 10067 semblait être plus stable thermiquement que l'enzyme produite par C. cinereus.Mots clés : espèce Coprinus, traitement à l'urée, phénol, traitement des eaux usées.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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