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Thermodependence of growth and enzymatic activities implicated in pathogenicity of two Erwinia carotovora subspecies (Pectobacterium spp.)

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Abstract:

Erwinia carotovora subsp. atroseptica and Erwinia carotovora subsp. carotovora can cause substantial damage to economically important plant crops and stored products. The occurrence of the disease and the scale of the damage are temperature dependent. Disease development consists first of active multiplication of the bacteria in the infection area and then production of numerous extracellular enzymes. We investigated the effects of various temperatures on these two steps. We assayed the specific growth rate and the pectate lyase and protease activities for eight strains belonging to E. carotovora subsp. atroseptica and E. carotovora subsp. carotovora in vitro. The temperature effect on growth rate and on pectate lyase activity is different for the two subspecies, but protease activity appears to be similarly thermoregulated. Our results are in agreement with ecological data implicating E. carotovora subsp. atroseptica in disease when the temperature is below 20 °C. The optimal temperature for pathogenicity appears to be different from the optimal growth temperature but seems to be a compromise between this temperature and temperatures at which lytic activities are maximal.Key words: temperature, Pectobacterium atrosepticum, Pectobacterium carotovorum, growth, pectate lyases, proteases.

Erwinia carotovora subsp. atroseptica et Erwinia carotovora subsp. carotovora provoquent des dégâts substantiels à des cultures économiquement importantes et aux récoltes stockées. L'apparition de la maladie et l'étendue des dégâts sont dépendantes de la température. Le développement de la maladie consiste tout d'abord en une multiplication active des bactéries dans la zone d'infection suivie d'une production de nombreuses enzymes extracellulaires. Nous avons étudié les effets de différentes températures sur ces deux étapes. Nous avons mesuré le taux de croissance spécifique et les activités de pectate lyase et protéase pour huit souches appartenant à E. carotovora subsp. atroseptica et à E. carotovora subsp. carotovora in vitro. L'effet de la température sur le taux de croissance et sur l'activité pectate lyase est différent pour les deux sous-espèces, mais l'activité protéase semble être pareillement thermo-régulée. Nos résultats sont en accord avec les données écologiques impliquant E. carotovora subsp. atroseptica dans la maladie quand la température est en-dessous de 20 °C. La température optimale pour la pathogénie apparaît être différente de la température optimale de croissance mais semble être un compromis entre cette température et les températures auxquelles les activités lytiques sont maximales.Mots clés : température, Pectobacterium atrosepticum, Pectobacterium carotovorum, croissance, pectate lyases, protéases.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2004

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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