Phenotypic characteristics and population genetics of Enterococcus faecalis cultured from patients in Tehran during 2000–2001

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Abstract:

Conventional bacteriology techniques were used to identify enterococci isolates cultured from patients at different hospitals in Tehran during 2000–2001. The identification was confirmed using species-specific PCR targeting the D-alanyl-D-alanine ligase gene. A total of 59 isolates of Enterococcus faecalis were identified. The rates of resistance to different antibiotics were in the following order: penicillin 84%, ciprofloxacin 42%, high-level gentamicin 30%, nitrofurantoin 14%, imipenem 4%, and chloramphenicol 2%. Resistance to ampicillin was found to be rare among the Iranian isolates of E. faecalis. Multi-locus enzyme electrophoresis was then used to analyze the strains. Forty-five electrophoretic types were obtained when 10 enzyme loci were screened. Although the collection of bacterial isolates was limited in time and location, considerable heterogeneity was found. Analysis of strains for linkage disequilibrium demonstrated that the studied population is not clonal, since the index of association was not significantly different from zero (Ia = 0.0296). Enterococcus faecalis isolates recovered from patients in Tehran were genetically diverse and seemed to possess a high potential for genetic recombinations, though none were resistant to vancomycin.Key words: Enterococcus faecalis, population genetics, MEE analysis, nosocomial infections.

Nous avons employé en premier lieu des techniques conventionnelles de bactériologie afin d'identifier des isolats d'entérocoques cultivés provenant de patients de divers hôpitaux de Téhéran au cours de 2000–2001. Les identifications ont été confirmées à l'aide d'un PCR spécifique à l'espèce ciblant le gène de la D-alanyl-D-alanine ligase. Un total de 59 isolats de Enterococcus faecalis ont été identifiés. Le taux de résistance à différents antibiotiques fut dans l'ordre : pénicilline 84 %, ciprofloxacine 42 %, gentamicine de haut niveau 30 %, nitrofurantoine 14 %, imipenem 4 % et chloramphénicol 2 %. La résistance à l'ampicilline fut rarement signalée chez les isolats iraniens de E. faecalis. Une électrophorèse enzymatique multilocus fut ensuite employée pour l'analyse des souches. Quarante-cinq types électrophorétiques ont été obtenus lorsque 10 locus d'enzymes ont été criblés. Bien que la collection d'isolats bactériens était limitée dans le temps et l'emplacement, une hétérogénéité considérable fut constatée. L'analyse des souches pour un déséquilibre de liaison a démontré que la population étudiée n'était pas clonale puisque l'index d'association ne différait pas significativement de zéro (Ia = 0,0296). Les isolats de E. faecalis recueillis de patients de Téhéran sont génétiquement diversifiés et semblent posséder un potentiel élevé de recombinaisons génétiques, bien qu'aucun ne soit résistant à la vancomycine.Mots clés : Enterococcus faecalis, génétique de populations, analyse par EEM, infections nosocomiales.

Document Type: Research Article

Publication date: October 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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