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Virulence of Candida albicans mutants toward larval Galleria mellonella (Insecta, Lepidoptera, Galleridae)

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Culture medium affected the virulence of a strain of Candida albicans toward Galleria mellonella larvae, but the yeast growth rates in yeast extract – peptone – dextrose broth and synthetic Galleria serum were not correlated with yeast virulence. Virulent C. albicans grew rapidly in larval serum, whereas, it limited nodulation and continued development in vivo, producing toxins that damaged the hemocytes and fat body. Nonpathogenic yeast-phase cells grew slowly in larval serum but induced extensively melanized nodules in vivo and developed no further. There was no discernible relationship in 14 exo-enzymes between the virulent and avirulent yeast strains and virulence. The avirulent myosin-I-defective yeast cells were rapidly removed from the hemolymph in vivo because of lysozyme-mediated yeast agglutination and the possible binding of the yeast cells by lysozyme and apolipophorin-III. Both lysozyme and apolipophorin-III are proteins that bind -1,3-glucan. Finally, insects with nonpathogenic C. albicans exhibited induced immunity and were more resistant to candidiasis from the wild-type yeast cells than were noninduced insects.Key words: Candida, virulence, insect, nodule, melanization, apolipophorin-III.

Le milieu de culture a affecté la virulence d'une souche de Candida albicans chez la larve de Galleria mellonella mais les taux de croissance des levures dans un bouillon levure – peptone – dextrose et dans un milieu synthétique de Galleria n'étaient pas corrélés avec leur virulence. Les C. albicans virulents poussaient rapidement en milieu larvaire. In vivo, elles ont limité la nodulation et ont continué leur développement tout en produisant des toxines qui ont endommagé les hémocytes et le corps gras. Des cellules non-pathogènes en phase de levure ont poussé lentement en milieu larvaire, ont induit des nodules fortement mélanisés in vivo et n'ont pas poursuit leur développement. Aucun rapport apparent n'a été noté suite à la comparaison du profil de 14 exo-enzymes des deux souches et virulences de levure. Les levures avirulentes déficientes en myosine I ont été rapidement éliminées de l'hémolymphe in vivo par l'action du lysozyme induisant une agglutination des levures et possiblement par la liaison au lysozyme et à l'apolipophorine-III. Le lysozyme et l'apolipophorine-III sont tous deux des protéines se liant au -1,3-glucane. Finalement, les insectes exposés à un C. albicans non-pathogène ont démontré une immunité induite et étaient plus résistants à la candidose par la levure sauvage que les insectes non induits.Mots clés : Candida, virulence, insecte, nodule, mélanisation, apolipophorine-III.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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