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A low molecular weight enterotoxic hemolysin from clinical Enterobacter cloacae

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Abstract:

Seven of 50 Enterobacter cloacae strains from clinical isolates produced small turbid zones of hemolysis in horse and sheep blood agar plates, and the culture supernatants were also positive for hemolytic activity. The hem o l y sin was partially purified from the culture supernatant of E. cloacae by ultrafiltration (PM-10 membrane) and extraction with acetone. Semipurified hemolysin was stable to heating (100 °C, 30 min) and was soluble in organic solvents (acetone, ethanol, and methanol). The toxin showed no loss of biological activity after treatment with trypsin and was stable to acid treatment at pH 2.0 but not at a pH greater than 7.0. In the rat intestinal loop assay, the hemolysin caused hemorrhagic fluid accumulation and severe histological alterations. These findings indicate that this hemolysin may be a putative virulence factor in E. cloacae infections.Key words: Enterobacter cloacae, hemolysin, enterotoxin.

Sept souches de Enterobacter cloacae sur 50 provenant d'isolats cliniques ont produit des zones troubles d'hémolyse sur des géloses au sang de cheval et de mouton, et les surnageants de culture étaient également positifs pour l'activité hémolytique. L'hémolysine a été partiellement purifiée du surnageant de culture de E. cloacae par ultrafiltration (membrane PM-10) et extraite à l'acétone. L'hémolysine partiellement purifiée était résistante au chauffage (100 °C, 30 min) et était soluble dans des solvants organiques (acétone, éthanol, méthanol). La toxine n'a démontré aucune perte d'activité biologique suite à un traitement à la trypsine, et était résistante à une traitement acide de pH 2,0, mais non à un pH supérieur à 7,0. À l'aide de l'essai de la boucle intestinale de rat, il fut constaté que l'hémolysine provoquait une accumulation de fluide hémorragique et des changements histologiques prononcés. Ces découvertes indiquent que cette hémolysine pourrait être une facteur de virulence putatif lors d'infections par E. cloacae.Mots clés : Enterobacter cloacae, hémolysine, entérotoxine.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: July 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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