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High TNT-transforming activity by a mixed culture acclimated and maintained on crude-oil-containing media

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A mixed microbial culture originating from a petroleum-contaminated site and maintained on crude oil exhibited high 2,4,6-trinitrotoluene (TNT) transformation activity. Cultivation of the mixed culture in glucose-containing medium for 29 h resulted in almost complete transformation of 100 ppm TNT. TNT transformation was observed with both growing and resting cells. With subculturing, it was found that TNT could support growth of the mixed culture when supplied as sole carbon source, sole nitrogen source, or sole carbon and nitrogen source. The finding that a mixed microbial culture maintained on crude oil exhibited high TNT transformation activity without prior subculture on TNT-containing media is novel and may have potential practical applications in the bioremediation of munitions-contaminated soil and wastewater.Key words: 2,4,6-trinitrotoluene, mixed culture, transformation, co-metabolism, surfactant.

Une culture microbienne mixte provenant d'un site contenant du pétrole et maintenue dans du pétrole brut a démontré une activité élevée de transformation du 2,4,6-trinitrotoluène (TNT). Une incubation de la culture mixte dans un milieu renfermant du glucose pendant 29 h a entraîné une transformation quasi complète de 100 ppm de TNT. La transformation du TNT a été observé avec des cellules en croissance ou au repos. Lors des repiquages, nous avons constaté que le TNT pouvait soutenir la croissance de la culture mixte lorsqu'il était fourni comme seule source de carbone, seule source d'azote, ou seule source de carbone et d'azote. Le fait qu'une culture microbienne mixte maintenue dans du pétrole brut démontre une activité élevée de transformation du TNT, sans repiquage préalable dans un milieu contenant du TNT, représente une découverte notoire. Elle aurait des applications pratiques potentielles dans l'assainissement biologique de sols et d'eaux usées contaminés aux munitions.Mots clés : 2,4,6-trinitrotoluène, culture mixte, transformation, cométabolisme, surfactant.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2003-05-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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