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Degradation of phenanthrene and naphthalene by a Burkholderia species strain

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Burkholderia sp. TNFYE-5 was isolated from soil for the ability to grow on phenanthrene as sole carbon and energy source. Unlike most other phenanthrene-degrading bacteria, TNFYE-5 was unable to grow on naphthalene. Growth substrate range experiments coupled with the ring-cleavage enzyme assay data suggest that TNFYE-5 initially metabolizes phenanthrene to 1-hydroxy-2-naphthoate with subsequent degradation through the phthalate and protocatechuate and -ketoadipate pathway. A metabolite in the degradation of naphthalene by TNFYE-5 was isolated by high-pressure liquid chromatography (HPLC) and was identified as salicylate by UV-visible spectral and gas chromatography – mass spectrometry analyses. Thus, the inability to degrade salicylate is apparently one major reason for the incapability of TNFYE-5 to grow on naphthalene.Key words: Burkholderia, phenanthrene, naphthalene, phthalate, protocatechuate.

La souche TNFYE-5 de l'espèce Burkholderia a été isolée du sol pour sa capacité à croître sur le phénanthrène comme seule source de carbone et d'énergie. Contrairement à la majorité des autres bactéries capables de dégrader le phénantrène, la souche TNFYE-5 était incapable de pousser sur le naphtalène. Des essais de croissance avec une gamme de substrats combinés avec les résultats d'essais de clivage enzymatique de l'anneau laissent croire que TNFYE-5 métabolise d'abord le phénanthrène en 1-hydroxy-2-naphtoate qui est ensuite dégradé par le sentier du phthalate, du protocatéchuate et du -cétoadipate. Un des métabolites de la dégradation du naphtalène par TNFYE-5 a été isolé par chromatographie liquide à haute performance (HPLC) et il a été identifié comme du salicylate par GC-MS et UV dans le spectre visible. L'incapacité de dégrader le salicylate est apparemment une des principales raisons expliquant l'inaptitude de la souche TNFYE-5 à croître sur le naphtalène.Mots cles : Burkholderia, phénanthrène, naphtalène, phthalate, protocatéchuate.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: February 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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