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Isolation and taxonomic and catabolic characterization of a 3,6-dimethylphenanthrene-utilizing strain of Sphingomonas sp.

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Abstract:

A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacterial biomass concomitant with a decrease in 3,6-DMP in a liquid mineral medium with this compound as its sole source of carbon and energy. Strain JS1 showed a high specificity in the use of the most abundant alkylderivatives of crude oils, such as alkylnaphthalenes and other alkylphenanthrenes, as the sole source of carbon and energy. It can also use several polycyclic aromatic hydrocarbons of three and four rings and their alkylated derivatives as growth substrates or transform them. The identification of several intermediate metabolites points to extensive metabolic activity, including the following: (i) aromatic ring oxidation and cleavage, (ii) methyl group oxidations, and (iii) methylenic oxidations. The metabolic actions of Sphingomonas sp. JS1 on the aromatic fraction extracted from a creosote-contaminated soil are also examined.Key words: dimethylphenanthrene, alkylated PAHs, biodegradation, Sphingomonas.

Nous avons isolé une souche de bactérie capable d'employer le 3,6-diméthylphénanthrène (3,6-DMP) en tant qu'unique source de carbone à partir d'un sol contaminé au créosote. Nous avons identifié l'isolat comme étant une souche de Sphingomonas sp. et l'avons nommé « souche JS1 ». La consommation du 3,6-DMP a été révélée par une augmentation de la biomasse bactérienne contaminante associée à une diminution de 3,6-DMP dans une milieu liquide minéral contenant ce composé en tant que seule source de carbone et d'énergie. La souche JS1 a démontré un spécificité élevée dans sa consommation des dérivés alkylés de pétroles bruts les plus abondants tels que les alkylnaphthalènes et autres alkylphénanthrènes, en tant qu'uniques sources de carbone et d'énergie. Elle peut également employer plusieurs hydrocarbures aromatiques polycycliques faits de trois ou quatre noyaux ainsi que leurs dérivés alkylés en tant que support de croissance, ou encore transformer ceux-ci. Les différents intermédiaires métaboliques identifiés signalent une activité métabolique diversifiée, incluant (i) l'oxydation et le clivage d'anneaux aromatiques, (ii) l'oxydation de groupes méthyles et (iii) l'oxydations de méthylènes. Nous avons également analysé les actions métaboliques de Sphingomonas sp. JS1 sur la fraction aromatique d'un sol contaminé au créosote.Mots clés : diméthylphénanthrène, HAP alkylés, biodégradation, Sphingomonas.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: February 1, 2003

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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