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Utilization of monocrotophos as phosphorus source by Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL 11

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Abstract:

Monocrotophos (dimethyl (E)-1-methyl-2-(methylcarbamoyl) vinyl phosphate, or MCP), an organophosphorus insecticide, was used as a sole phosphorus source by the microorganisms isolated from the soil. None of the isolates could utilize MCP as a sole source of carbon. Two of the potential microbial isolates, Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL 11, could utilize MCP as a sole source of phosphorus. Pseudomonas aeruginosa F10B showed a lag phase of 4 h, while in the case of C. michiganense subsp. insidiosum SBL 11, it was 8 h when cultured in the presence of MCP. The generation time for both strains was increased in the medium containing MCP. It was 2.15 h for P. aeruginosa F10B in MCP medium as compared with 1.29 h in basal medium, while in case of C. michiganense subsp. insidiosum SBL 11 it was increased to 3.4 h in MCP medium as compared with 1.28 h in basal medium. These two strains were able to degrade technical MCP in shake-flask culture up to 98.9 and 86.9%, respectively, and pure MCP up to 79 and 80%, respectively, within 24 h at 37°C. The optimal concentration of MCP required for the normal growth was 500 ppm. In the substrate preference study, Tris–p-nitrophenyl phosphate was the most preferred substrate followed by paraoxon. The enzyme responsible for the break down of MCP was phosphotriesterase, which was localized on the membrane-bound fraction of the disrupted cells. The gene responsible for the production of phosphotriesterase (opd) in P. aeruginosa F10B was plasmid-borne.Key words: biodegradation, monocrotophos, phosphotriesterase, P. aeruginosa, C. michiganense subsp. insidiosum.

Le monocrotophos (diméthyl (E)-1 méthyl-2-(méthylcarbinol) vinyl phosphate), MCP, un insecticide organophosphoré, a été utilisé comme seule source de phosphore par des microorganismes isolés du sol. Aucun de ces isolats ne pouvait utiliser le MCP comme seule source de carbone. Deux de ces isolats microbiens potentiels, Pseudomonas aeruginosa F10B et Clavibacter michiganense subsp. insidiosum SBL 11 pouvaient utiliser le MCP comme seule source de phosphore. En culture en présence de MCP, P. aeruginosa F10B affichait une phase de latence de 4 h, alors que dans le cas de C. michiganense subsp. insidiosum SBL 11, cette phase était de 8 h. Le temps de génération augmentait dans le milieu contenant du MCP. Dans le milieu avec MCP, le temps de génération du P. aeruginosa F10B était de 2, 15 h comparativement à 1, 29 h dans le milieu de base alors que dans le cas de C. michiganense subsp. insidiosum SBL11, le temps de génération augmentait à 3, 4 h dans le milieu MCP comparativement à 1, 28 h dans le milieu de base. Ces deux souches étaient capables de dégrader du MCP (grade technique) en culture dans des flacons avec agitation jusqu'à 98,9 et 86,9 % respectivement et du MCP pur jusqu'à 79 et 80 % respectivement en 24 h à 37° C. La concentration optimale de MCP pour assurer une croissance normale était de 500 ppm. Dans une étude de préférence de substrats, le meilleur substrat était le Tris–p-nitrophényl phosphate suivi du paraoxon. L'enzyme responsable de la dégradation du MCP était la phosphotriestérase localisée dans la fraction liée à la membrane de cellules éclatées. Le gène responsable (opd) de la production de la phosphotriestérase chez P. aeruginosa F10B était localisé sur un plasmide.Mots clés : biodégradation, monocrotophos, phosphotriestérase, P. aeruginosa, C. michiganense subsp. insidiosum.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: February 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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