Algicidal activity and gliding motility of Saprospira sp. SS98-5

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Abstract:

A marine bacterium, Saprospira sp. SS98-5, which was isolated from Kagoshima Bay, Japan, was able to kill and lyse the cells of the diatom Chaetoceros ceratosporum. The multicellular filamentous cells of this bacterium captured the diatom cells, formed cell aggregates, and lysed them in an enriched sea water (ESS) liquid medium. Strain SS98-5 also formed plaques on double layer agar plates incorporating diatom cells. The diatom cell walls were partially degraded at the contact sites with the bacteria, the bacteria invaded from there into the diatom cells, and then the diatom cells were completely lysed. The strain possessed gliding motility and grew as spreading colonies on ESS agar plates containing lower concentrations of polypeptone (below 0.1%) while forming nonspreading colonies on ESS agar plates containing 0.5% polypeptone. Electron micrographs of ultrathin sections demonstrated that microtubule-like structures were observable only in gliding motile cells. Both the gliding motility and the microtubule-like structures were diminished by the addition of podophyllotoxin, an inhibitor of microtubule assembly, suggesting that the microtubule-like structures observed in these bacterial cells are related to their gliding motility.Key words: Saprospira sp., Chaetoceros ceratosporum, gliding motility, algicidal activity, microtubule-like structure.

La souche SS98-5 de Saprospira sp. est une bactérie marine isolée de la Baie de Kagoshima au Japon. Ce microorganisme est capable de lyser et de tuer la diatomée Chaetoceros ceratosporum. Les cellules filamenteuses de cette bactérie sont capables de capter les diatomées, de former des agrégats cellulaires et de causer leur lyse dans un milieu liquide ESS. La souche SS98-5 entraîne la formation de plages sur des géloses à double couche contenant des diatomées incorporées. Les parois de cellules de diatomées étaient partiellement dégradées au point de contact avec les bactéries qui par la suite envahissaient les cellules de diatomées à partir de ce site et finalement lysaient totalement les cellules de diatomées. Cette souche présentait une mobilité par glissement et formait des colonies envahissantes sur des géloses ESS qui contenaient des concentrations faibles de polypeptone (moins de 0,1 %), mais les colonies n'étaient plus envahissantes sur des géloses contenant 0,5 % de polypeptone. Les micrographies électroniques de coupes minces ont permis d'observer des structures en forme de tubules uniquement chez les cellules mobiles par glissement. Cette mobilité par glissement et les structures en forme de microtubules étaient réduites lors de l'addition de podophyllotoxine, un inhibiteur de l'assemblage des microtubules. Ces observations suggèrent que les structures en forme de microtubules observées chez ces cellules bactériennes seraient apparentées à la mobilité par glissement.Mots clés : Saprospira sp.; Chaaetoceros ceratosporus; mobilité par glissement, activité algicide; structure en forme de microtubule.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: February 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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