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Effect of genotype and root colonization in biological control of fusarium wilts in pigeonpea and chickpea by Pseudomonas aeruginosa PNA1

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Pseudomonas aeruginosa PNA1, an isolate from chickpea rhizosphere in India, protected pigeonpea and chickpea plants from fusarium wilt disease, which is caused by Fusarium oxysporum f.sp. ciceris and Fusarium udum. Inoculation with strain PNA1 significantly reduced the incidence of fusarium wilt in pigeonpea and chickpea on both susceptible and moderately tolerant genotypes. However, strain PNA1 protected the plants from fusarium wilt until maturity only in moderately tolerant genotypes of pigeonpea and chickpea. Root colonization of pigeonpea and chickpea, which was measured using a lacZ-marked strain of PNA1, showed tenfold lower root colonization of susceptible genotypes than that of moderately tolerant genotypes, indicating that this plant–bacteria interaction could be important for disease suppression in this plant. Strain PNA1 produced two phenazine antibiotics, phenazine-1-carboxylic acid and oxychlororaphin, in vitro. Its Tn5 mutants (FM29 and FM13), which were deficient in phenazine production, caused a reduction or loss of wilt disease suppression in vivo. Hence, phenazine production by PNA1 also contributed to the biocontrol of fusarium wilt diseases in pigeonpea and chickpea.Key words: biocontrol, fusarium wilts, phenazines, Pseudomonas.

La souche de Pseudomonas aeruginosa PNA1, isolée en Inde de la rhizosphère du pois chiche, est capable de protéger les plants de pois chiches et de pois cajans contre la flétrissure à fusarium causée par Fusarium oxysporum f.sp. ciceris et Fusarium udum. L'inoculation avec la souche PNA1 a diminué significativement l'incidence de la flétrissure à fusarium chez des génotypes du pois chiche et du pois cajan sensibles ou modérément résistants. La souche PNA1 n'a par contre protégé jusqu'à maturité que les plants modérément résistants de pois chiche et de pois cajan. La colonisation racinaire du pois chiche et du pois cajan évaluée avec une souche PNA1 porteuse du gène lacZ était de dix fois inférieure chez les génotypes sensibles comparativement aux génotypes modérément tolérants, ce qui suggère que l'interaction bactérie–plante pourrait être importante pour éliminer cette maladie chez cette plante. In vitro, la souche PNA1 produit deux antibiotiques du groupe phénazine, soit la phénazine-1-acide carboxylique et l'oxychlororaphine. Des mutants de cette souche Tn5 (FM29 et FM13) déficients en production de phénazine ont perdu complètement ou ont vu réduire leur capacité de supprimer la flétrissure. Nous en concluons que la phénazine produite par PNA1 contribue aussi au biocontrôle des maladies à fusarium chez le pois chiche et le pois cajan.Mots clés : biocontrôle, flétrissure à fusarium, phénazines, Pseudomonas.[Traduit par la Rédaction]

Keywords: biocontrol, fusarium wilts, phenazines, Pseudomonas

Document Type: Research Article

Publication date: February 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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