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Characterization of a clinical Vibrio cholerae O139 isolate from Mexico

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Abstract:



Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDRE1) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenotypic characteristics of this strain were identical to those of V. cholerae O1 biotype El Tor. Unlike ctxA-positive V. cholerae O139 strains, this strain was sensitive to a wide panel of antibiotics, including ampicillin, chloramphenicol, ciprofloxacin, gentamicin, furazolidone, nalidixic acid, nitrofurantoin, tetracycline, trimethoprim-sulfamethoxazole, and streptomycin, but was resistant to polymyxin B. Ribotype and pulsed-field gel electrophoresis profiles of INDRE1 differed from those of ctxA-positive V. cholerae O139 and other V. cholerae strains. Phenotypic characteristics of the Mexico strain were similar to those reported for V. cholerae O139 isolates from Argentina and Sri Lanka.Key words: Vibrio cholerae O139, cholera toxin, ctxA, tcpA.

Les souches pathogènes de Vibrio cholereae 0139 possèdent le gène de la sous-unité A de la toxine du choléra (ctxA) ainsi que le gène des pili corégulés par la toxine (tcpA). Nous avons isolé une souche de V. cholereae 0139 (INDRE1) ctxA négative et tcpA négative chez un patient à Mexico atteint d'une infection gastrointestinale. Certaines des caractéristiques phénotypiques de cette souche étaient identiques à celles de V. cholereae 01 du biotype El Tor. Contrairement aux souches de V. cholereae 0139 ctxA positives, cette souche était sensible à un large éventail d'antibiotiques dont la l'ampicilline, le chloramphénicol, la ciprofloxacine, la gentamicime, le furazolidone, l'acide nalidixique, le nitrofurantoin, la tétracycline, le triméthoprim sulfaméthaxaxole et la streptomycine mais elle était résistance à la polymixine B. Le ribotype et les profils observés par électrophorèse en champ pulsé de INDRE1 étaient différents des V. cholereae 0139 ctxA positifs et des autres souches de V. cholereae. Les caractéristiques phénotypiques de la souche Mexico étaient semblables à celles des isolats de V. cholereae 0139 provenant de l'Argentine et du Sri Lanka.Mots cles : Vibrio cholereae 0139, toxine du choléra, ctxA, tcpA.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2003-01-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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