Quantification and diversity of the archaeal community in a landfill site

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Abstract:



At a sea-based, solid waste disposal site, methanogenic organisms were quantified by molecular approaches. The samples collected for analysis were from anaerobic leachate of the landfill site. When the DNA extracted from the leachate was examined by a quantitative PCR method using domain-specific 16S rDNA primers, archaeal DNA represented 2–3% of the total extracted DNA. On the basis of cloning and sequence comparison of the archaeal PCR products, more than half of the sequences belonged to Euryarchaeota, particularly relatives of the genus Methanosaeta. The cloning analysis suggested that the majority of methane emitted from the landfill site originated from the acetate-utilizing Methanosaeta.Key words: landfill, methanogen, archaea, 16S rDNA.

Nous avons voulu quantifier, selon une approche moléculaire, les organismes méthanogènes présents dans un lieu d'enfouissement maritime de déchets solides. Les échantillons servant à cette étude ont été récoltés du lixiviat anaérobie du lieu d'enfouissement. Après analyse de l'ADN extrait du lixiviat par une méthode PCR utilisant des amorces d'ADNr 16S spécifiques de domaines, nous avons constaté que l'ADN des archaea représentait 2–3 % de l'ADN total extrait. En comparant, les essais de clonage et les séquences des produits PCR des archaea, nous avons constaté que la moitié des séquences appartenaient aux Euryarchaeota particulièrement apparentés au genre Methanosaeta. L'analyse du clonage suggère que la plus forte proportion du méthane émis au lieu d'enfouissement provenait des Methanosaeta utilisateurs d'acétate.Mots clés : lieu d'enfouissement, méthanogène, archaea, ADNr 16S.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2003

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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