Enhancement of population densities of fluorescent pseudomonads in the rhizosphere of tomato plants by addition of acibenzolar-S-methyl

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Abstract:



Fluorescent pseudomonad isolates G309 and CW2, in combination with the resistance inducer acibenzolar-S-methyl (ASM), improved control of fungal and bacterial diseases on tomato plants. The interactions of the bacteria in the presence of ASM showed that in vitro growth of Pseudomonas fluorescens G309 and Pseudomonas sp. strain CW2 was not affected in King's B broth supplemented with 10 and 20 mM ASM. Also, the bacterial cells were not able to utilize ASM as a nutrient source. In vitro production of the two antimicrobial secondary metabolites phenazine-1-carboxylic acid and 2-OH-phenazine by the isolate CW2 was not affected within 3 days from incubation. In contrary, addition of ASM at a concentration of 20 mM to King's B liquid medium significantly increased production of salicylic acid by isolate G309. When roots of tomato plants were treated with G309 or CW2 cell suspensions containing 20 mM ASM, the number of bacterial cells recovered from the rhizosphere was significantly higher in the combined treatments than in the single applications 5, 10, and 15 days after inoculation. However, ASM at a higher concentration (50 mM) did not appreciably enhance the population sizes of either bacterial isolate in the rhizosphere. Enhanced bacterial cell densities in the rhizosphere of tomato plants were also determined following simultaneous treatments of tomato roots with 10 and 20 mM ASM in combination with the transformed isolate G309-384 (mini-Tn5gfp), which encodes the green fluorescent protein.Key words: acibenzolar-S-methyl, fluorescent pseudomonads, green fluorescent protein, tomato.

Les isolats de pseudomonades G309 et CW2 combinés à l'inducteur de résistance acibenzolar-S-methyl (ASM) ont amélioré le contrôle de maladies de la tomate causées par des champignons ou des bactéries. Les interactions de la bactérie en présence d'ASM ont indiqué qu'un bouillon de culture King's B enrichi avec 10 et 20 mM d'ASM n'a pas affecté la croissance in vitro de la souche G309 de Pseudomonas fluorescens et de la souche CW2 de Pseudomonas sp. De plus, les bactéries ont été incapables de consommer l'ASM comme nutriment. La production in vitro des deux métabolites secondaires antimicrobiens, l'acide phenazine-1-carboxylique et la 2-OH-phenazine, par l'isolat CW2 n'a pas été influencé au cours des 3 jours d'incubation. En revanche, l'ajout d'ASM à un milieu liquide King's B à une concentration de 20 mM a augmenté significativement la production d'acide salicylique par l'isolat G309. Lorsque des racines de plants de tomate ont été traitées avec des suspensions de cellules G309 ou CW2 renfermant 20 mM d'ASM, le nombre de bactéries isolées de nouveau à partir de la rhizosphère à la suite d'un traitement combiné fut significativement supérieur à celui obtenu à la suite d'applications simples, 5, 10 ou 15 jours après l'inoculation. Cependant, une concentration d'ASM plus élevée (50 mM) n'a pas substantiellement accru les tailles des populations des deux isolats bactériens dans la rhizosphère. Des densités accrues de bactéries dans la rhizosphère de plants de tomate ont également été décelées à la suite de traitements simultanés de racines de tomates avec 10 et 20 mM d'ASM combinés avec l'isolat transformé G309-384 (mini-Tn5gfp), qui code la protéine verte fluorescente.Mots clés : acibenzolar-S-methyl, pseudomonades fluorescentes, protéine verte fluorescente, tomate.[Traduit par la Rédaction]

Keywords: acibenzolar-S-methyl; fluorescent pseudomonads; green fluorescent protein; protéine verte fluorescente; pseudomonades fluorescentes; tomate; tomato

Document Type: Research Article

Publication date: December 1, 2002

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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