The pathway of dephosphorylation of myo-inositol hexakisphosphate by phytate-degrading enzymes of different Bacillus spp.

Authors: Greiner, R.; Farouk, A.; Alminger, M.L.; Carlsson, N-G.

Source: Canadian Journal of Microbiology, Volume 48, Number 11, November 2002 , pp. 986-994(9)

Publisher: NRC Research Press

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Abstract:



The pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytate-degrading enzymes of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and Bacillus amyloliquefaciens 45 was established using a combination of high-performance ion chromatography analysis and kinetic studies. The data demonstrate that all the Bacillus phytate-degrading enzymes under investigation dephosphorylate myo-inositol hexakisphosphate by sequential removal of phosphate groups via two independent routes; the routes proceed via D-Ins(1,2,4,5,6)P5 to Ins(2,4,5,6)P4 to finally Ins(2,4,6)P3 or D-Ins(2,5,6)P3 and via D-Ins(1,2,4,5,6)P5 to D-Ins(1,2,5,6)P4 to finally D-Ins(1,2,6)P3. The resulting myo-inositol trisphosphate D-Ins(1,2,6)P3 was degraded via D-Ins(2,6)P2 to finally Ins(2)P after prolonged incubation times in combination with increased enzyme concentration. Key words: Bacillus spp., myo-inositol phosphate isomers, phytase, phytate degradation.

Nous avons reconstitué la voie de déphosphorylation du myo-inositol hexakisphosphate par les enzymes dégradant le phytate qu'on retrouve chez Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, et Bacillus amyloliquefaciens 45 à l'aide d'analyses de chromatographie ionique à haute performance et d'études de cinétique. Les données démontrent que toutes les enzymes de Bacillus étudiées dégradant le phytate pouvaient déphosphoryler le myo-inositol hexakisphosphate par l'enlèvement séquentiel de groupes phosphate et ce, par le biais de deux voies indépendantes: les voie passent par le D-Ins(1,2,4,5,6)P5, le Ins(2,4,5,6)P4 pour aboutir au Ins(2,4,6)P3 ou au D-Ins(2,5,6)P3, et par le D-Ins(1,2,4,5,6)P5, le D-Ins(1,2,5,6)P4 pour aboutir au D-Ins(1,2,6)P3. Le myo-inositol trisphosphate D-Ins(1,2,6)P3 résultant a été dégradé en du Ins(2)P en passant par le D-Ins(2,6)P2, à la suite de temps d'incubation prolongés combinés à une concentration plus élevée d'enzyme.Mots clés : Bacillus spp., isomères de myo-inositol phosphate, phytase, dégradation du phytate.[Traduit par la Rédaction]

Keywords: Bacillus spp

Document Type: Research Article

Publication date: November 1, 2002

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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