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Development of a selective myclobutanil agar (MBA) medium for the isolation of Fusarium species from asparagus fields

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A new selective myclobutanil agar medium for the detection of Fusarium species is proposed. Ten media formulations based on various selective agents (pentachloronitrobenzene (PCNB), Rose Bengal, malachite green, sodium hypochlorite, captan, benomyl, chlorotalonil, myclobutanil, thiram, and cupric sulfate) were compared. First, mycelium growth and colony appearance of Alternaria alternata, Aspergillus flavus, Cladosporium cladosporioides, Epicoccum nigrum,Fusarium sp., Fusarium solani, Fusarium moniliforme, Fusarium oxysporum f.sp. dianthi, Penicillium sp., and Trichoderma viride isolates were compared. Second, the ability of the different media to isolate and enumerate fusaria from asparagus fields was evaluated. The myclobutanil-based medium showed the highest selectivity to Fusarium spp. growth but required a slightly longer incubation time (>5 d) than peptone–pentachloronitrobenzene-based agar (PPA) (< 5 d). PPA allowed a faster fusaria growth but also permited the growth of other moulds. The other media were less selective and did not allow to isolate fusaria or to differenciate them from other growing fungi.Key words: selective medium, myclobutanil, Fusarium, soil, Asparagus.

Nous proposons un nouveau milieu sélectif au myclobutanil (MBA) pour la détection des espèces de Fusarium. Dix formulations basées sur différents ingrédients sélectifs (pentachloronitrobenzene (PCNB), Rose Bengal, vert de malachite, hypochlorite de sodium, captan, benomyl, chlorotalonil, myclobutanil, thiram et sulfate de cuivre) ont été évaluées. Premièrement, la croissance du mycélium et l'apparence des colonies d'isolats d'Alternaria alternata, Aspergillus flavus, Cladosporium cladosporioides, Epicoccum nigrum, Fusarium sp., Fusarium solani, Fusarium moniliforme, Fusarium oxysporum f.sp. dianthi, Penicillium sp. et de Trichoderma viride ont été comparées. Deuxièmement, la capacité des différents milieux à isoler et permettre le dénombrement des Fusarium spp. à partir de champs d'asperges a été évaluée. Le milieu à base de myclobutanil a montré la plus grande sélectivité pour la croissance des Fusarium mais il nécessitait une période d'incubation légèrement plus longue (>5 j) que le milieu gélosé à base de pentachloronitrobenzène et de peptone (PPA) (<5 j). Par contre, le PPA a permis une croissance des Fusarium plus rapide mais il a permis aussi la croissance d'autres champignons. Les autres milieux se sont avérés moins efficaces pour isoler sélectivement les Fusarium et permettre leur distinction des autres espèces de champignons croissant simultanément.Mots clés : milieu sélectif, myclobutanil, Fusarium, sol, Asparagus.

Keywords: Asparagus; Fusarium; milieu sélectif; myclobutanil; selective medium; soil; sol

Document Type: Research Article

Publication date: 2002-09-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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