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Protein analysis in a pleomorphically deteriorated strain of the insect-pathogenic fungus Metarhizium anisopliae

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Abstract:

Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on agar. Gel electrophoresis was used to examine differences in intracellular protein patterns (urea-soluble proteins and urea-insoluble proteins (i.e., hydrophobins)) in conidiating and mycelial cultures of M. anisopliae. Two major proteins present in a conidiating culture and one from a mycelial culture were N-terminally sequenced but showed no homologies to known proteins. The presence of hydrophobins in conidiating and mycelial cultures was also examined, and it was shown that these proteins were abundant in conidiating cultures but not in mycelial cultures. We also used primers designed from regulatory genes involved in conidiation in Aspergillus nidulans. The amplified fragments were not homologous to A. nidulans genes.Key words: Metarhizium anisopliae, conidia, pleomorphic deterioration, protein analysis.

La détérioration pléomorphique est une processus par lequel une isolat fongique perd la capacité de produire des conidies suite à repiquages répétés. Nous avons isolé précédemment des souches du champignon entomopathogène Metarhizium anisopliae qui ont perdu de façon irréversible leur capacité à produire des conidies et qui produisent seulement du mycélium lorsque cultivés sur agar. Nous avons utilisé l'électrophorèse sur gel afin d'analyser les différences dans les patrons de protéines intracellulaires (protéines solubles dans l'urée et protéines insolubles, c.-à-d. les hydrophobines) dans des cultures conidiantes et mycéliales de M. anisopliae. Deux protéines majeures présentes dans une culture conidiante et une provenant d'une culture mycéliale ont été séquencées en N-terminal, mais n'ont démontré aucune homologie avec des protéines connues. De plus, nous avons évalué la présence d'hydrophobines dans des cultures conidiantes et mycéliales et avons révélé que ces protéines étaient abondantes dans les cultures conidiantes mais non dans les cultures mycéliales. Nous avons également utilisé des amorces conçues à partir de gènes régulateurs impliqués dans la conidiation de Aspergillus nidulans. Les fragments amplifiés n'étaient pas homologues à des gènes de A. nidulans.Mots clés : Metarhizium anisopliae, conidies, détérioration pléomorphique, analyse de protéines.[Traduit par la Rédaction]

Keywords: Metarhizium anisopliae; analyse de protéines; conidia; conidies; détérioration pléomorphique; pleomorphic deterioration; protein analysis

Document Type: Research Article

Publication date: September 1, 2002

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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