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Cultivation-dependent characterization of bacterial diversity from British Columbia forest soils subjected to disturbance

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Abstract:

Bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (FAME) analysis. Soil samples from a British Columbia Ministry of Forests Long-Term Soil Productivity (LTSP) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot N) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot S)) and from an unlogged reference plot (REF). Seventy-five percent of 1795 bacterial isolates were affiliated with 42 genera representing - and -Proteobacteria, Actinobacteria, the Bacillus/Clostridium group, and the Cytophaga-Flexibacter-Bacteroides group. Approximately half of the culture collection represented genetic diversity confined to four bacterial genera: Pseudomonas, Bacillus, Paenibacillus, and Arthrobacter. A significantly higher proportion of bacterial isolates belonging to Actinobacteria, and the member genus Arthrobacter, were isolated from plot S soil samples compared with soil samples from plots N and REF. Twenty-five percent of bacterial isolates were not conclusively identified to genus with FAME analysis. MIDI Tracker cluster analysis and partial 16S rRNA gene sequence analysis enabled classification of a subset of these isolates.Key words: bacterial diversity, fatty acid methyl ester analysis (FAME), forest soil.

Des bactéries retrouvées dans de la matière organique de surface et dans des horizons de sols minéralisées forestiers ont été cultivées à l'aide de quatre méthodes et caractérisées par une analyse des dérivés d'esters méthyliques d'acides gras (FAME). Les échantillons de sol provenant d'une installation de Productivité des Sols à Long Terme (LTSP) du ministère des Forêts de la Colombie-Britannique ont été recueillis pendant l'hiver et l'été à la suite de deux traitements perturbateurs(une récolte d'arbres entiers sans compactage du sol (terrains N) et une récolte d'arbres entiers plus un enlèvement complet de la matière organique de surface accompagné d'un compactage du sol lourd (terrains S)) et à partir d'un terrain de référence non coupé (REF). Soixante-quinze pour cent de 1795 isolats bactériens ont été rattachés à 42 genres appartenant aux groupes des - et -Proteobacteria, des Actinobacteria, de Bacillus/Clostridium, et de Cytophaga-Flexibacter-Bacteroides. Environ la moitié de la collection de cultures était le reflet de la diversité génétique restreinte à quatre genres de bactéries, soit Pseudomonas, Bacillus, Paenibacillus et Arthrobacter. Une proportion significativement plus élevée d'isolats bactériens appartenant à Actinobacteria, et au genre Arthobacter s'y rattachant, a été récupérée des échantillons de terrains S comparativement aux échantillons de sol des terrains N et REF. Il fut impossible d'accorder un genre à25% des isolats bactériens par l'analyse FAME. Une analyse de groupement MIDI Tracker et un séquençage partiel du gène de l'ARN 16s a permis de classifier une portion de ces isolats.Mots clés : diversité bactérienne, analyse des dérivés d'esters méthyliques d'acides gras (FAME), sols forestiers.

Keywords: analyse des dérivés d'esters méthyliques d'acides gras (FAME); bacterial diversity; bactérienne; diversité; fatty acid methyl ester analysis (FAME); forest soil; sols forestiers

Document Type: Research Article

Publication date: July 1, 2002

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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