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Peptidases affecting recombinant protein production by Streptomyces lividans

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The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.Key words: fermentation, interleukin-3, Streptomyces, tripeptidyl peptidase, proteases.

L'influence des peptidases sur la production d'interleukine-3 (rhIL-3) par un Streptomyces lividans recombinant a été étudiée. La bactérie a produit quelques peptidases générales et des tripeptidyl peptidases compromettant l'authenticité de la rhIL-3. Le taux de peptidases était dépendant de la morphologie de croissance. Il fut possible de diminuer l'activité des peptidases en cultivant S. lividans sous forme de culots compacts. L'activité peptidase générale maximale dans les cultures en culots a été repoussée après que la concentration maximale de rhIL-3 eut été atteinte. L'activité de la tripeptidyl peptidase était associée au produit (rhIL-3).Mots clés : fermentation, interleukine-3, Streptomyces, tripeptidyl peptidases, protéases.[Traduit par la Rédaction]

Keywords: Streptomyces; fermentation; interleukin-3; interleukine-3; proteases; protéases; tripeptidyl peptidase; tripeptidyl peptidases

Document Type: Research Article

Publication date: 2001-12-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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