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Isolation of an esterase-producing Trichosporon brassicae and its catalytic performance in kinetic resolution of ketoprofen

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A yeast strain CGMCC 0574, identified as Trichosporon brassicae, was selected from 92 strains for its high (S) selectivity in the hydrolysis of ketoprofen ethyl ester. The effective strains of the microorganisms were isolated from soil samples with the ester as the sole carbon source. The ethyl ester proved to be the best substrate for resolution of ketoprofen among several ketoprofen esters examined. The resting cells of CGMCC 0574 could catalyze the hydrolysis of ketoprofen ethyl ester with an enantiomeric ratio of 44.9, giving (S)-ketoprofen an enantiomeric excess of 91.5% at 42% conversion.Key words: ketoprofen, biocatalytic resolution, enantioselective hydrolysis, microbial esterase, Trichosporon brassicae.

Une souche de levure désignée CGMCC 0574, identifiée comme étant Trichosporon brassicae, a été sélectionnée parmi 92 souches sur la base de sa (S)-sélectivité élevée lors de l'hydrolyse de l'ester éthylique de ketoprofen. La sélection s'est effectuée sur des souches de micro-organismes ayant été isolées à partir d'échantillons de sol avec l'ester comme seule source de carbone. L'ester éthylique s'est avéré être le meilleur substrat pour la résolution du ketoprofen parmi plusieurs esters de ketoprofen analysés. Les cellules de CGMCC 0574 au repos ont pu catalyser l'hydrolyse de l'ester éthylique de ketoprofen avec un rapport énantiomérique de 44,9, produisant du (S)-ketoprofen à 91,5 % excès énantiomérique, à un taux de conversion de 42 %.Mots clés : ketoprofen, résolution biocatalytique, hydrolyse énantio-sélective, estérases microbiennes, Trichosporon brassicae.[Traduit par la Rédaction]

Keywords: Trichosporon brassicae; biocatalytic resolution; enantioselective hydrolysis; estérases microbiennes; hydrolyse énantio-sélective; ketoprofen; microbial esterase; résolution biocatalytique

Document Type: Research Article

Publication date: December 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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